Changes on histone H3 modifications on the GLUT5 gene and its expression in Caco-2 cells co-treated with a p44/42 MAPK inhibitor and glucocorticoid hormone

被引：8
作者：
Mochizuki, Kazuki [1 ]
Takabe, Satsuki [1 ]
Goda, Toshinao [1 ]
机构：
[1] Univ Shizuoka, Lab Nutrit Physiol, Grad Sch Nutrit & Environm Sci & Global COE, Shizuoka 4228526, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 02期
关键词：
glucocorticoid hormone; histone modification; GLUT5; p44/42; MAPK; Caco-2; cells;
D O I：
10.1016/j.bbrc.2008.04.075
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Changes on histone H3 modifications from methylation at lysine 9 to acetylation at lysine 9/14 is thought to be important for transcriptional activation of genes associated with differentiation In this study, we found that the treatment with a p44/42 MAPK inhibitor PD98059 induced di-methylation at lysine 9 on the upstream/transcriptional regions of the GLUT5 gene. Co-treatment of PD98059 with a glucocorticoid hormone agonist, dexamethasone (Dex), not only repressed the induction of di-methylation by PD98059, but also enhanced the acetylation of histone H3 at lysine 9114 on the GLUT5 gene, as well as its gene expression. These results suggest that the histone H3 di-methylation at lysine 9, as well as acetylation at lysine 9/14, may be indispensable for coordinated induction of the GLUT5 gene by p44/42 MAP kinase inhibition and the glucocorticoid hormone. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：324 / 327
页数：4
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