Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein

被引：35

作者：

Tan, Fu-Rong ^{[1
]}

Dai, Li-Chun ^{[1
]}

Wu, Bo ^{[1
]}

Qin, Han ^{[1
]}

Shui, Zong-Xia ^{[1
]}

Wang, Jing-Li ^{[1
]}

Zhu, Qi-Li ^{[1
]}

Hu, Qi-Chun ^{[1
,2
]}

Ruan, Zhi-Yong ^{[3
]}

He, Ming-Xiong ^{[1
,2
]}

机构：

[1] Minist Agr, Biogas Inst, Biomass Energy Technol Res Ctr, Sect 4 13, Chengdu 610041, Peoples R China

[2] Minist Agr, Key Lab Dev & Applicat Rural Renewable Energy, Chengdu 610041, Peoples R China

[3] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 99卷 / 12期

关键词：

Furfural tolerance; Ethanol production; Global transcription machinery engineering; Error-prone PCR; Sigma factor; RpoD protein; ESCHERICHIA-COLI; ETHANOL-PRODUCTION; RNA-POLYMERASE; TRANSCRIPTION MACHINERY; FERMENTATION; STRAIN; ACID; XYLOSE; STRATEGIES; INHIBITORS;

D O I：

10.1007/s00253-015-6577-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Furfural from lignocellulosic hydrolysates is the key inhibitor for bio-ethanol fermentation. In this study, we report a strategy of improving the furfural tolerance in Zymomonas mobilis on the transcriptional level by engineering its global transcription sigma factor (sigma(70), RpoD) protein. Three furfural tolerance RpoD mutants (ZM4-MF1, ZM4-MF2, and ZM4-MF3) were identified from error-prone PCR libraries. The best furfural-tolerance strain ZM4-MF2 reached to the maximal cell density (OD600) about 2.0 after approximately 30 h, while control strain ZM4-rpoD reached its highest cell density of about 1.3 under the same conditions. ZM4-MF2 also consumed glucose faster and yield higher ethanol; expression levels and key Entner-Doudoroff (ED) pathway enzymatic activities were also compared to control strain under furfural stress condition. Our results suggest that global transcription machinery engineering could potentially be used to improve stress tolerance and ethanol production in Z. mobilis.

引用

页码：5363 / 5371

页数：9

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