Neuroprotective effects of chlorogenic acid against apoptosis of PC12 cells induced by methylmercury

被引：89

作者：

Li, Yongjin ^{[1
,2
]}

Shi, Wei ^{[3
]}

Li, Yindong ^{[4
]}

Zhou, Yong ^{[2
]}

Hu, Xuefeng ^{[1
]}

Song, Chunmei ^{[2
]}

Ma, Hongbo ^{[2
]}

Wang, Changwen ^{[2
]}

Li, Yong ^{[1
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Dept Food Hyg & Nutr, Beijing 100083, Peoples R China

[2] Jilin Med Coll, Sch Publ Hlth, Jilin 132013, Peoples R China

[3] Jilin Med Coll, Sch Nursing, Jilin 132013, Peoples R China

[4] Shunyi Ctr Dis Control & Prevent, Beijing 101300, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2008年 / 26卷 / 01期

关键词：

chlorogenic acid; neurotoxicity; methylmercury; free radicals; PC12; cells;

D O I：

10.1016/j.etap.2007.12.008

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Chlorogenic acid (CGA) widely exists in edible and medicinal plants. We aimed to evaluate the effect of CGA on the protection from apoptosis by methylmercury (MeHg) in PC12 cells. Cell viability was evaluated by MTT assay. Apoptosis was assayed by flow cytometry detection. Caspase-3 activity was measured by confocal microscopy. Intracellular GSH levels were determined by bicinchoninic acid protein assay. Intracellular reactive oxygen species (ROS) was assessed by means of chloromethyl-dihydrodichlorofluorescein diacetate. Glutathione peroxidase (GPx) activity was determined by UV. In order to elucidate the action of CGA, the protective effects of CGA were compared to Vit.E. CGA was effective at protecting PC 12 cells against MeHg-induced damage in dose-dependent manner. CGA not only suppressed the generation of ROS, the decrease of activity in GPx and the decrease of GSH, but also attenuated caspase-3 activation in PC 12 cells by MeHg. CGA eventually protected PC 12 cells against MeHg-induced apoptosis. The results highlighted that CGA may exert neuroprotective effects through its antioxidant actions. (C) 2008 Published by Elsevier B.V.

引用

页码：13 / 21

页数：9

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