Patterns of surface immobilized block copolymer vesicle nanoreactors

被引：11

作者：

Chen, Qi ^{[1
]}

de Groot, G. Wilhelmina ^{[1
]}

Schonherr, Holger ^{[1
]}

Vancso, G. Julius ^{[1
]}

机构：

[1] Univ Twente, Dept Mat Sci & Technol Polymers, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

来源：

EUROPEAN POLYMER JOURNAL | 2011年 / 47卷 / 02期

关键词：

Block copolymer vesicles; Soft lithography; Micro-molding in capillaries; Enzyme and substrates; POLYMERIZED LIPID VESICLES; POLYDIACETYLENE SUPRAMOLECULES; MICROARRAYS;

D O I：

10.1016/j.eurpolymj.2010.11.005

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The immobilization and positioning of ultra small reaction vessels on solid supports open new pathways in applications such as lab-on-a-chip, sensors, microanalyses and microreactors. In our work block copolymer vesicles made from polystyrene-block-polyacrylic acid (PS-b-PAA) were immobilized from aqueous medium onto 3-amino propyl trimethoxysilane functionalized silicon surfaces exploiting electrostatic interactions. The immobilization of the vesicles was investigated by Fourier transform infrared (FTIR) spectroscopy, as well as fluorescence optical and atomic force microscopy (AFM). In addition, the influence of pH and ionic strength on the surface coverage of vesicles bound to the surface was elucidated. Finally micro-molding in capillaries (MIMIC) was utilized to create line patterns of the vesicles containing the enzyme trypsin and the fluorogenic substrate rhodamine 110 bisamide. The selective positioning of vesicle nanoreactors in conjunction with electrostatic immobilization serves as a proof of principle for potential applications in real-time observation of confined chemical reaction inside vesicles as nanocontainers and for the fabrication of integrated microarray systems. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：130 / 138

页数：9

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