Quantitative proteomics using the high resolution accurate mass capabilities of the quadrupole-orbitrap mass spectrometer

被引:18
作者
Gallien, Sebastien [1 ]
Domon, Bruno [1 ]
机构
[1] CRP Sante, Luxembourg Clin Prote Ctr LCP, Strassen, Luxembourg
关键词
COMPLEX PEPTIDE MIXTURES; TARGETED PROTEOMICS; QUANTIFICATION; ACQUISITION; FRAGMENTATION; SELECTIVITY; DISCOVERY; PROTEINS; BIOLOGY; MS/MS;
D O I
10.4155/bio.14.115
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High resolution/accurate mass hybrid mass spectrometers have considerably advanced shotgun proteomics and the recent introduction of fast sequencing capabilities has expanded its use for targeted approaches. More specifically, the quadrupole-orbitrap instrument has a unique configuration and its new features enable a wide range of experiments. An overview of the analytical capabilities of this instrument is presented, with a focus on its application to quantitative analyses. The high resolution, the trapping capability and the versatility of the instrument have allowed quantitative proteomic workflows to be redefined and new data acquisition schemes to be developed. The initial proteomic applications have shown an improvement of the analytical performance. However, as quantification relies on ion trapping, instead of ion beam, further refinement of the technique can be expected.
引用
收藏
页码:2159 / 2170
页数:12
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