Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

被引：32

作者：

Maria Rocha-Ramirez, Luz ^{[1
]}

Hernandez-Ochoa, Beatriz ^{[2
]}

Gomez-Manzo, Said ^{[3
]}

Marcial-Quino, Jaime ^{[4
]}

Cardenas-Rodriguez, Noemi ^{[5
]}

Centeno-Leija, Sara ^{[6
]}

Garcia-Garibay, Mariano ^{[7
]}

机构：

[1] Hosp Infantil Mexico Dr Federico Gomez, Unidad Invest Enfermedades Infecciosas, Secretaria Salud Dr Marquez 162, Ciudad De Mexico 06720, Mexico

[2] Hosp Infantil Mexico Dr Federico Gomez, Lab Inmunoquim & Biol Celular, Secretaria Salud Dr Marquez 162, Ciudad De Mexico 06720, Mexico

[3] Inst Nacl Pediat, Lab Bioquim Genet, Secretaria Salud, Ciudad De Mexico 04530, Mexico

[4] Consejo Nacl Ciencia & Tecnol CONACYT, Secretaria Salud, Inst Nacl Pediat, Ciudad De Mexico 04530, Mexico

[5] Inst Nacl Pediat, Lab Neurociencias, Secretaria Salud, Ciudad De Mexico 04530, Mexico

[6] Univ Colima, Consejo Nacl Ciencia & Tecnol CONACYT, Tecnoparque CLQ, Lab Agrobiotecnol, Carretera Los Limones Loma de Juarez, Colima 28629, Mexico

[7] Univ Autonoma Metropolitana, Dept Ciencias Alimentac, Unidad Lerma, Av San Rafael Atlixco 186 Col Vicentina, Ciudad De Mexico 09340, Mexico

来源：

MICROORGANISMS | 2020年 / 8卷 / 01期

关键词：

probiotic; Lactobacillus casei IMAU60214; health; innate immunity; macrophage; Toll-like receptors; LACTIC-ACID BACTERIA; DENDRITIC CELLS; CYTOKINE; IMMUNITY; BETA; VIVO; PEPTIDOGLYCAN; INTERLEUKIN-8; INFLAMMATION; ENHANCEMENT;

D O I：

10.3390/microorganisms8010079

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Most Lactobacillus species have beneficial immunological ("immunoprobiotic") effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-beta) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain.

引用

页数：13

共 62 条

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