Cloud point extraction of salicylic acid from human urine and its spectrofluorimetric determination

被引:0
作者
Tabrizi, Ahad Bavili [1 ]
机构
[1] Tabriz Univ Med Sci, Fac Pharm, Dept Med Chem, Tabriz, Iran
[2] Tabriz Univ Med Sci, Biotechnol Res Ctr, Tabriz, Iran
来源
CHEMIA ANALITYCZNA | 2007年 / 52卷 / 05期
关键词
salicylic acids; cloud point extraction; spectrofluorimetry; human urine;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Salicylic acid (SA) has been extracted from human urine using cloud point extraction and determined applying spectrofluorimetry. Experimental conditions, such as concentrations of HCl and Triton X-114, temperature, and equilibration time have been adjusted to optimize the extraction process and to obtain analytical characteristics of the method. Linearity was observed in the analyte's concentration range of 0.025-2.5 mg L-1. Detection limit of the method was 0.011 mg L-1. Relative standard deviation for 5 replicate determinations of SA at the 1.0 mg L-1 concentration level was 1.85%. Good recoveries in the range of 85-94% were obtained for the spiked samples. The method was applied to the determination of SA in human urine.
引用
收藏
页码:823 / 833
页数:11
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