Structural evolution of supported lipid bilayers intercalated with quantum dots

被引:6
作者
Wlodek, Magdalena [1 ]
Slastanova, Anna [2 ]
Fox, Laura J. [2 ]
Taylor, Nicholas [2 ]
Bikondoa, Oier [3 ,4 ]
Szuwarzynski, Michal [5 ]
Kolasinska-Sojka, Marta [1 ]
Warszynski, Piotr [1 ]
Briscoe, Wuge H. [2 ]
机构
[1] Polish Acad Sci, Jerzy Haber Inst Catalysis & Surface Chem, Niezapominajek 8, PL-30239 Krakow, Poland
[2] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England
[3] European Synchrotron ESRF, UK CRG Beamline, XMaS, 71 Ave Martyrs, F-38043 Grenoble, France
[4] Univ Warwick, Dept Phys, Gibbet Hill Rd, Coventry CV4 7AL, W Midlands, England
[5] AGH Univ Sci & Technol, Acad Ctr Mat & Nanotechnol, Al A Mickiewicza 30, PL-30059 Krakow, Poland
基金
英国工程与自然科学研究理事会;
关键词
Supported lipid bilayers; X-ray reflectivity (XRR); Nanoparticle-membrane interactions; Membranes intercalated with quantum dots; Bilayer structure; POLYELECTROLYTE MULTILAYERS; PHOSPHOLIPID-BILAYERS; MODEL; NANOPARTICLES; MEMBRANE; PHOSPHATIDYLETHANOLAMINE; FUSION; ADSORPTION; PROTEINS; PATHWAY;
D O I
10.1016/j.jcis.2019.11.102
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Hypothesis: Supported lipid bilayers (SLBs) embedded with hydrophobic quantum dots (QDs) undergo temporal structural rearrangement. Experiments: Synchrotron X-ray reflectivity (XRR) was applied to monitor the temporal structural changes over a period of 24 h of mixed SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) / 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine (POPE) intercalated with 4.9 nm hydrophobic cadmium sulphide quantum dots (CdS QDs). The QD-embedded SLBs (QD-SLBs) were formed via rupture of the mixed liposomes on a positively charged polyethylene imine (PEI) monolayer. Atomic force microscopy (AFM) imaging provided complementary characterization of the bilayer morphology. Findings: Our results show time-dependent perturbations in the SLB structure due to the interaction upon QD incorporation. Compared to the SLB without QDs, at 3 h incubation time, there was a measurable decrease in the bilayer thickness and a concurrent increase in the scattering length density (SLD) of the QD-SLB. The QD-SLB then became progressively thicker with increasing incubation time, which - along with the fitted SLD profile - was attributed to the structural rearrangement due to the QDs being expelled from the inner leaflet to the outer leaflet of the bilayer. Our results give unprecedented mechanistic insights into the structural evolution of QD-SLBs on a polymer cushion, important to their potential biomedical and biosensing applications. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码:409 / 417
页数:9
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