The imminent role of protein engineering in synthetic biology

被引：48

作者：

Foo, Jee Loon ^{[1
]}

Ching, Chi Bun ^{[1
]}

Chang, Matthew Wook ^{[1
]}

Leong, Susanna Su Jan ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore

来源：

BIOTECHNOLOGY ADVANCES | 2012年 / 30卷 / 03期

关键词：

Protein engineering; Synthetic biology; Metabolic engineering; Enzyme; UNNATURAL AMINO-ACIDS; ESCHERICHIA-COLI; DIRECTED EVOLUTION; GENETIC-CODE; BACTERIAL PHOSPHOTRIESTERASE; STREPTOMYCES-AVERMITILIS; BIOSYNTHETIC-PATHWAY; EXPANDING METABOLISM; STRUCTURE PREDICTION; FEEDBACK INHIBITION;

D O I：

10.1016/j.biotechadv.2011.09.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein engineering has for decades been a powerful tool in biotechnology for generating vast numbers of useful enzymes for industrial applications. Today, protein engineering has a crucial role in advancing the emerging field of synthetic biology, where metabolic engineering efforts alone are insufficient to maximize the full potential of synthetic biology. This article reviews the advancements in protein engineering techniques for improving biocatalytic properties to optimize engineered pathways in host systems, which are instrumental to achieve high titer production of target molecules. We also discuss the specific means by which protein engineering has improved metabolic engineering efforts and provide our assessment on its potential to continue to advance biology engineering as a whole. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：541 / 549

页数：9

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