The attractive recombinant phytase from Bacillus licheniformis: biochemical and molecular characterization (vol 98, pg 5937, 2014)

被引：15

作者：

Borgi, Mohamed Ali ^{[1
]}

Boudebbouze, Samira ^{[2
]}

Mkaouar, Hela ^{[2
]}

Maguin, Emmanuelle ^{[2
]}

Rhimi, Moez ^{[2
]}

机构：

[1] Fac Sci Gafsa, Unit Macromol Biochem & Genet, Dept Life Sci, Zarroug, Gafsa, Tunisia

[2] INRA, UMR Micalis 1319, Jouy En Josas, France

来源：

BIOENGINEERED | 2015年 / 6卷 / 04期

关键词：

Bacillus licheniformis; biotechnological applications; enzyme engineering; phytase; protein biochemistry; BETA-PROPELLER PHYTASE; FUNGAL PHYA GENE; CLONING; ACID; EXPRESSION; PERFORMANCE; LEVEL; YOUNG; GEL;

D O I：

10.1080/21655979.2015.1048050

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phytases catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol (having important role in metabolism and signal transduction pathways), and inorganic phosphate. These enzymes have been widely used in animal feed in order to improve phosphorus nutrition and to decrease pollution in animal waste. Compared to previously described phytases, the phytase (PhyL) from Bacillus licheniformis ATCC 14580 has attractive biochemical properties which can increase the profitability of several biotechnological procedures (animal nutrition, humain health...etc). Due to its amino acid sequence with critical substitutions, the PhyL could be a model to enhance other phytases features, in terms of thermal stability and high activity. Otherwise, an engineered PhyL, with low pH optimum, will represent a challenge within the class of beta- propeller phytases.

引用

页码：233 / 236

页数：4

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