Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin

被引：2
|
作者：
Clerté, S
Dautant, A
d'Estaintot, BL
Gallois, B
Mizunoe, Y
Wai, SN
Precigoux, G
机构：
[1] Univ Bordeaux 1, UPRES A 5471, Unite Biophys Struct, F-33405 Talence, France
[2] Kyushu Univ, Fac Med, Dept Bacteriol, Higashi Ku, Fukuoka 812, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S090744499801004X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The prokaryotic ferritin gene of Campylobacter jejuni was overexpressed in Escherichia coli under control of the bacteriophage T7 promoter and the protein (Cj-FTN) purified. Preliminary crystallization experiments have been performed using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. Diffraction studies show the crystals belong to the I432 space group (a = 151.52 Angstrom). Structure solution by molecular replacement is in progress while crystal quality improvement is carried out.
引用
收藏
页码：299 / 301
页数：3
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