Occurrence of Toxoplasma gondii and Hammondia hammondi oocysts in the faeces of cats from Germany and other European countries

被引：109

作者：

Schares, G. ^{[1
]}

Vrhovec, A. Globokar ^{[2
]}

Pantchev, N. ^{[2
]}

Herrmann, D. C. ^{[1
]}

Conraths, F. J. ^{[1
]}

机构：

[1] Inst Epidemiol, Fed Res Inst Anim Hlth, Friedrich Loeffler Inst, D-16868 Wusterhausen, Germany

[2] Vet Med Labor GmbH, Div IDEXX Labs, D-71636 Ludwigsburg, Germany

来源：

VETERINARY PARASITOLOGY | 2008年 / 152卷 / 1-2期

关键词：

Toxoplasma gondii; Hammondia hammondi; oocyst; genotyping; PCR;

D O I：

10.1016/j.vetpar.2007.12.004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Faecal samples of 24,106 cats from Germany and other European countries were examined microscopically in a veterinary laboratory in Germany between October 2004 and November 2006 to estimate the prevalence of animals shedding Toxoplasma gondii or Hammondia hammondi oocysts. Oocysts of 9-15 mu m size with a morphology similar to that of H. hammondi and T. gondii were found in 74 samples (0.31%). A total of 54 samples were further characterised to achieve a species diagnosis and to determine the genotype of T. gondii isolates by PCR and PCR-RFLP. From these samples, 48 isolates were obtained: 26 (0.11%) were finally identified as T. gondii and 22 (0.09%) as H. hammondi. T. gondii-positive samples came from Germany, Austria, France and Switzerland while H. hammondi was detected in samples from Germany, Austria and Italy. In two samples (one T. gondii and one H. hammondi), PCR indicated the presence of Hammondia heydorni DNA. No Neospora caninum DNA was detected in any of the feline faecal samples. Twenty-two of the 26 T. gondii isolates could be genotyped. A PCR-RFLP analysis for the SAG2, SAG3, GRA6 and BTUB genes revealed T. gondii genotype II in all cases. Morphologically, H. hammondi oocysts exhibited a statistically significantly smaller Length-Width-Ratio than T. gondii oocysts. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：34 / 45

页数：12

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