SYBR®Green qPCR methods for detection of endogenous reference genes in commodity crops: a step ahead in combinatory screening of genetically modified crops in food and feed products

被引：23

作者：

Mbella, E. Guillaume Mbongolo ^{[1
]}

Lievens, Antoon ^{[1
]}

Barbau-Piednoir, Elodie ^{[1
]}

Sneyers, Myriam ^{[1
]}

Leunda-Casi, Amaya ^{[1
]}

Roosens, Nancy ^{[1
]}

Van den Bulcke, Marc

机构：

[1] Sci Inst Publ Hlth, B-1050 Brussels, Belgium

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2011年 / 232卷 / 03期

关键词：

GMO; SYBR (R) Green; Endogenous reference gene; GM crop; Real-time PCR; ENGL; Soybean; Maize; Oilseed rape; Rice; Cotton; Sugar beet; QUANTITATIVE PCR DETECTION; QUANTIFICATION; VALIDATION; MAIZE; GMOS;

D O I：

10.1007/s00217-010-1408-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Identification of crops present in food and/or feed matrices represents an important step in the screening strategies targeting genetically modified organisms (GMO). Soybean, maize, oilseed rape, rice, cotton, sugar beet and potato are to date the most important sources of genetically modified materials imported in the European Union (EU). In order to allow detection of their presence in an integrated screening approach, a set of SYBR (R) Green real-time polymerase chain reaction (qPCR) methods has been developed which can be used under the same assay conditions and at similar efficiency for each of the abovementioned crops. Each qPCR method is shown to meet the performance criteria (i.e. specificity, limit of detection and PCR efficiency) set by the European Network of GMO Laboratories (ENGL). When combined with the equivalent qPCR methods targeting GMO elements, these crop-specific SYBR (R) Green qPCR methods can aid the development of an efficient tool for determining GMO presence in food and/or feed products.

引用

页码：485 / 496

页数：12

共 28 条

[1]

Arumuganathan K., 1991, Plant Mol Biol Rep, V9, P208, DOI [10.1007/BF02672069, DOI 10.1007/BF02672069]

[2] SYBRA®Green qPCR screening methods for the presence of "35S promoter" and "NOS terminator" elements in food and feed products [J].

Barbau-Piednoir, Elodie ;

Lievens, Antoon ;

Mbongolo-Mbella, Guillaume ;

Roosens, Nancy ;

Sneyers, Myriam ;

Leunda-Casi, Amaya ;

Van den Bulcke, Marc .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2010, 230 (03) :383-393

[3]

BERBEN G, 2001, TRACAGE AUTHENTIFICA

[4]

*CERA, CERAS DAT SAF INF WE

[5]

*COMM REF LAB, EV SPEC METH QUANT S

[6]

*COMM REF LAB, EV SPEC METH QUANT R

[7]

*COMM REF LAB, EV SPEC METH QUANT O

[8]

*COMM REF LAB, EV SPEC METH QUANT C

[9] NUCLEIC-ACID SEQUENCE-BASED AMPLIFICATION [J].

COMPTON, J .

NATURE, 1991, 350 (6313) :91-92

[10] GMDD: a database of GMO detection methods [J].

Dong, Wei ;

Yang, Litao ;

Shen, Kailin ;

Kim, Banghyun ;

Kleter, Gijs A. ;

Marvin, Hans J. P. ;

Guo, Rong ;

Liang, Wanqi ;

Zhang, Dabing .

BMC BIOINFORMATICS, 2008, 9 (1)

← 1 2 3 →