Inhibition of plasmalemmal Na+/Ca2+ exchange by mitochondrial Na+/Ca2+ exchange inhibitor 7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4,1-benzothiazepin-2(3H)-one (CGP-37157) in cerebellar granule cells

被引:32
作者
Czyz, A
Kiedrowski, L
机构
[1] Univ Illinois, Inst Psychiat, Dept Psychiat, Chicago, IL 60612 USA
[2] Univ Illinois, Inst Psychiat, Dept Pharmacol, Chicago, IL 60612 USA
关键词
NCX; NCKX; CGP-37157; KB-R7943; mitochondria; neurons;
D O I
10.1016/j.bcp.2003.08.024
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
In the heart, 7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4, 1-benzothiazepin-2(3H)-one (CGP-37157) inhibits mitochondrial but not sarcolemmal Na+/Ca2+ exchange. Therefore, CGP-37157 is often used as an experimental tool to study the role of mitochondrial Na+/Ca2+ exchange in Ca2+ homeostasis in various cells, including neurons. However, neurons express several K+-dependent (NCKX) and/or K+-independent (NCX) isoforms of plasmalemmal Na+/Ca2+ exchange not expressed in the sarcolemma. Because it has never been determined whether CGP-37157 inhibits plasmalemmal NCKX and/or NCX isoforms in neurons, we tested this possibility. As an index of NCKX and/or NCX activity, we studied Na-dependent and gramicidin-induced Ca-45(2+) accumulation in the presence and absence of K+, respectively. In primary cultures of cerebellar granule cells, CGP-37157 with IC50 of 13 muM inhibited over 70% of plasmalemmal NCX activity (P < 0.01) but not NCKX activity. Our data suggest that the effects of CGP-37157 on neuronal Ca2+ homeostasis include inhibition of certain plasmalemmal NCX isoform(s). Because cerebellar granule cells robustly express NCX3 transcripts, which are not expressed in the heart, it appears that this isoform may be CGP-37157 sensitive. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:2409 / 2411
页数:3
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