Bay laurel (Laurus nobilis) as potential antiviral treatment in naturally BQCV infected honeybees

被引：20

作者：

Aurori, Adriana C. ^{[1
,2
]}

Bobis, Otilia ^{[1
]}

Dezmirean, Daniel S. ^{[1
]}

Marghitas, Liviu A. ^{[1
]}

Erler, Silvio ^{[1
]}

机构：

[1] Univ Stiinte Agr & Med Vet, Dept Apicultura & Sericicultura, Calea Manastur 3-5, Cluj Napoca 400372, Romania

[2] Univ Babes Bolyai, Colectivul Ingn Genet Vegetala, Str Clin 5-7, Cluj Napoca 3400, Romania

来源：

VIRUS RESEARCH | 2016年 / 222卷

关键词：

BQCV; Honeybee; Antiviral activity; Plant secondary metabolite; Polyphenol; Flavonoid; PATHOGEN PAENIBACILLUS-LARVAE; COLONY COLLAPSE DISORDER; APIS-MELLIFERA; VARROA-DESTRUCTOR; VIRUS-INFECTIONS; ANTIBACTERIAL ACTIVITY; AMERICAN FOULBROOD; BEE COLONIES; IMMUNITY; EXTRACTS;

D O I：

10.1016/j.virusres.2016.05.024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viral diseases are one of the multiple factors associated with honeybee colony losses. Apart from their innate immune system, including the RNAi machinery, honeybees can use secondary plant metabolites to reduce or fully cure pathogen infections. Here, we tested the antiviral potential of Laurus nobilis leaf ethanolic extracts on forager honeybees naturally infected with BQCV (Black queen cell virus). Total viral loads were reduced even at the lowest concentration tested (1 mg/ml). Higher extract concentrations (>5 mg/ml) significantly reduced virus replication. Measuring vitellogenin gene expression as an indicator for transcript homeostasis revealed constant RNA levels before and after treatment, suggesting that its expression was not impacted by the L. nobilis treatment. In conclusion, plant secondary metabolites can reduce virus loads and virus replication in naturally infected honeybees. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：29 / 33

页数：5

共 49 条

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