Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9

被引：5

作者：

Lorthongpanich, Chanchao ^{[1
]}

Jiamvoraphong, Nittaya ^{[1
]}

Supakun, Prapasri ^{[1
]}

Damkham, Nattaya ^{[1
,2
]}

Terbto, Papussorn ^{[3
]}

Waeteekul, Supaporn ^{[4
]}

U-Pratya, Yaowalak ^{[1
,5
]}

Laowtammathron, Chuti ^{[1
]}

Issaragrisil, Surapol ^{[1
,5
,6
]}

机构：

[1] Mahidol Univ, Siriraj Hosp, Siriraj Ctr Excellence Stem Cell Res, Dept Med,Fac Med, Bangkok, Thailand

[2] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Immunol, Bangkok, Thailand

[3] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Pathol, Bangkok, Thailand

[4] Mahidol Univ, Siriraj Hosp, Fac Med, Div Med Genet,Dept Obstet & Gynaecol, Bangkok, Thailand

[5] Mahidol Univ, Siriraj Hosp, Fac Med, Div Hematol,Dept Med, Bangkok, Thailand

[6] Wattanosoth Hosp, BDMS Ctr Excellence Canc, Bangkok Hematol Ctr, Bangkok, Thailand

来源：

STEM CELL RESEARCH | 2019年 / 41卷

关键词：

D O I：

10.1016/j.scr.2019.101634

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

WWTR1 or TAZ (WWTR1/TAZ) is a transcriptional coactivator that acts as a downstream regulatory target in the Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation and anti-apoptosis. It has been shown in other cell types that WWTR1/TAZ plays a redundant role to its homolog YAP1. Using CRISPR/Cas9 gene editing, we established the WWTR1/TAZ-KO cell line, which features homozygous deletion of WWTR1 gene from human iPSCs. The established WWTR1/YAZ-KO cell line maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and normal karyotype.

引用

页数：4

共 4 条

[1] TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins [J].