Effects of Systemic or Local Administration of Mesenchymal Stem Cells from Patients with Osteoporosis or Osteoarthritis on Femoral Fracture Healing in a Mouse Model

被引:7
作者
Laguna, Esther [1 ]
Perez-Nunez, Maria Isabel [1 ]
del Real, Alvaro [2 ]
Menendez, Guillermo [1 ]
Sainz-Aja, Jose A. [3 ]
Lopez-Delgado, Laura [4 ]
Sanudo, Carolina [5 ]
Martin, Alicia [5 ]
Mazorra, Remigio [6 ]
Ferreno, Diego [3 ]
Garcia-Montesinos, Belen [7 ]
Riancho, Jose A. [4 ]
机构
[1] Univ Cantabria, Inst Invest Sanitaria Valdecilla IDIVAL, Hosp Univ Marques Valdecilla, Serv Traumatol & Cirugia Ortoped,Fac Med, Santander 39008, Spain
[2] Univ Cantabria, Inst Invest Sanitaria Valdecilla IDIVAL, Fac Med, Dept Med & Psiquiatria, Santander 39011, Spain
[3] Univ Cantabria, Lab Div Ciencia & Ingn Ios Mat LADICIM, Escuela Tecn Super Ingn Caminos Canales & Puertos, Santander 39005, Spain
[4] Univ Cantabria, Inst Inves Sanitaria Valdecilla IDIVAL, Hosp Univ Marques Valdecilla, Serv Med Interna,Fac Med, Santander 39011, Spain
[5] Univ Cantabria, Inst Invest Sanitaria Valdecilla IDIVAL, Fac Med, Santander 39011, Spain
[6] Hosp Univ Marques Valdecilla, Serv Anat Patol, Santander 39008, Spain
[7] Hosp Univ Marques Valdecilla, Serv Cirugia Maxilofacial, Santander 39008, Spain
关键词
mesenchymal stem cells; bone regeneration; animal model; micro-CT; histological study; BONE; PROMOTES; REPAIR;
D O I
10.3390/biom12050722
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this study was to analyze the regenerative capacity of mesenchymal stem cells (MSCs) in the treatment of fractures. MSCs extracted from patients with osteoporotic hip fractures or hip osteoarthritis undergoing hip replacement surgeries were cultured and injected into mice with femoral fracture. Two experimental models were established, one for the systemic administration of MSCs (n = 29) and another one for local administration (n = 30). Fracture consolidation was assessed by micro-CT and histology. The degree of radiological consolidation and corticalization was better with MSCs from osteoporosis than from osteoarthritis, being significant after systemic administration (p = 0.0302 consolidation; p = 0.0243 corticalization). The histological degree of consolidation was also better with MSCs from osteoporosis than from osteoarthritis. Differences in histological scores after systemic infusion were as follows: Allen, p = 0.0278; Huo, p = 0.3471; and Bone Bridge, p = 0.0935. After local administration at the fracture site, differences in histological scores were as follows: Allen, p = 0.0764; Huo, p = 0.0256; and Bone Bridge, p = 0.0012. As osteoporosis and control groups were similar, those differences depended on an inhibitory influence by MSCs from patients with osteoarthritis. In conclusion, we found an unexpected impairment of consolidation induced by MSCs from patients with osteoarthritis. However, MSCs from patients with osteoporosis compared favorably with cells from patients with osteoarthritis. In other words, based on this study and previous studies, MSCs from patients with osteoporosis do not appear to have worse bone-regenerating capabilities than MSCs from non-osteoporotic individuals of similar age.
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页数:12
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