A pathway for mitotic chromosome formation

被引：481

作者：

Gibcus, Johan H. ^{[1
]}

Samejima, Kumiko ^{[2
]}

Goloborodko, Anton ^{[3
,4
]}

Samejima, Itaru ^{[2
]}

Naumova, Natalia ^{[1
]}

Nuebler, Johannes ^{[3
,4
]}

Kanemaki, Masato T. ^{[5
,6
]}

Xie, Linfeng ^{[7
]}

Paulson, James R. ^{[7
]}

Earnshaw, William C. ^{[2
]}

Mirny, Leonid A. ^{[3
,4
]}

Dekker, Job ^{[1
,8
]}

机构：

[1] Univ Massachusetts, Dept Biochem & Mol Pharmacol, Program Syst Biol, Med Sch, 368 Plantation St, Worcester, MA 01605 USA

[2] Univ Edinburgh, Wellcome Ctr Cell Biol, Kings Bldg, Edinburgh EH9 3BF, Midlothian, Scotland

[3] MIT, Inst Med Engn & Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[4] MIT, Dept Phys, Cambridge, MA 02139 USA

[5] Res Org Informat & Syst, Div Mol Cell Engn, Natl Inst Genet, Yata 1111, Mishima, Shizuoka 4118540, Japan

[6] SOKENDAI, Dept Genet, Mishima, Shizuoka 4118540, Japan

[7] Univ Wisconsin, Dept Chem, 800 Algoma Blvd, Oshkosh, WI 54901 USA

[8] Univ Massachusetts, Howard Hughes Med Inst, Med Sch, 368 Plantation St, Worcester, MA 01605 USA

来源：

SCIENCE | 2018年 / 359卷 / 6376期

基金：

日本学术振兴会; 英国惠康基金; 美国国家科学基金会;

关键词：

SISTER-CHROMATID RESOLUTION; CONDENSIN-I; METAPHASE CHROMOSOMES; VERTEBRATE CELLS; TOPOISOMERASE-II; SCAFFOLD ATTACHMENT; CHROMOKINESIN KIF4A; MAMMALIAN-CELLS; LOOP EXTRUSION; HUMAN GENOME;

D O I：

10.1126/science.aao6135

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, similar to 80-kb inner loops are nested within similar to 400-kb outer loops. The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold. The size of helical turns progressively increases to similar to 12 megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding.

引用

页码：652 / +

页数：13

共 84 条

[1] The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II [J].