Continuous subcellular resolution three-dimensional imaging on intact macaque brain

被引:24
作者
Zhou, Can [1 ]
Yang, Xiaoquan [1 ,2 ]
Wu, Shihao [3 ]
Zhong, Qiuyuan [1 ]
Luo, Ting [1 ]
Li, Anan [1 ,2 ,4 ]
Liu, Guangcai [1 ]
Sun, Qingtao [2 ]
Luo, Pan [1 ]
Deng, Lei [1 ]
Ni, Hong [1 ]
Tan, Chaozhen [1 ]
Yuan, Jing [1 ,2 ]
Luo, Qingming [5 ]
Hu, Xintian [3 ,4 ]
Li, Xiangning [1 ,2 ]
Gong, Hui [1 ,2 ,4 ]
机构
[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, MoE Key Lab Biomed Photon, Wuhan 430074, Peoples R China
[2] JITRI, HUST Suzhou Inst Brainsmat, Suzhou 215123, Peoples R China
[3] Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China
[4] Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China
[5] Hainan Univ, Sch Biomed Engn, Haikou 570228, Hainan, Peoples R China
基金
中国国家自然科学基金;
关键词
Large-volume samples; Macaque; Continuous 3D imaging; Mesoscopic projectome; Single axonal resolution; CORTICOTHALAMIC PROJECTIONS; MEDIODORSAL THALAMUS; TRANSGENIC MICE; ATLAS; MRI; OPPORTUNITIES; GENERATION; ORGANS;
D O I
10.1016/j.scib.2021.08.003
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
To decipher the organizational logic of complex brain circuits, it is important to chart long-distance pathways while preserving micron-level accuracy of local network. However, mapping the neuronal projections with individual-axon resolution in the large and complex primate brain is still challenging. Herein, we describe a highly efficient pipeline for three-dimensional mapping of the entire macaque brain with subcellular resolution. The pipeline includes a novel poly-N-acryloyl glycinamide (PNAGA)based embedding method for long-term structure and fluorescence preservation, high-resolution and rapid whole-brain optical imaging, and image post-processing. The cytoarchitectonic information of the entire macaque brain was acquired with a voxel size of 0.32 gm x 0.32 gm x 10 gm, showing its anatomical structure with cell distribution, density, and shape. Furthermore, thanks to viral labeling, individual long-distance projection axons from the frontal cortex were for the first time reconstructed across the entire brain hemisphere with a voxel size of 0.65 gm x 0.65 gm x 3 gm. Our results show that individual cortical axons originating from the prefrontal cortex simultaneously target multiple brain regions, including the visual cortex, striatum, thalamus, and midbrain. This pipeline provides an efficient method for cellular and circuitry investigation of the whole macaque brain with individual-axon resolution, and can shed light on brain function and disorders. (c) 2021 Science China Press. Published by Elsevier B.V. and Science China Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:85 / 96
页数:12
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