The specificity of PCR-based protocols for detection of Erwinia amylovora

被引：28

作者：

Powney, Rachel ^{[1
,2
,3
,4
]}

Beer, Steven V. ^{[2
]}

Plummer, Kim ^{[3
]}

Luck, Jo ^{[1
,4
]}

Rodoni, Brendan ^{[1
,4
]}

机构：

[1] Cooperat Res Ctr Natl Plant Biosecur, Bruce, ACT 2617, Australia

[2] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA

[3] La Trobe Univ, Dept Bot, Latrobe, Vic 3086, Australia

[4] Dept Primary Ind, Knoxfield, Vic 3180, Australia

来源：

AUSTRALASIAN PLANT PATHOLOGY | 2011年 / 40卷 / 01期

关键词：

Fire blight; Diagnostics; Molecular identification; FIRE BLIGHT PATHOGEN; ESCHERICHIA-COLI; NESTED-PCR; IDENTIFICATION; PEAR; DIAGNOSIS; DISEASE; GROWTH; GENES; AGENT;

D O I：

10.1007/s13313-010-0017-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An evaluation of seven published conventional PCR protocols used for the detection of Erwinia amylovora has shown that six out of the seven protocols tested were not specific for all strains of E. amylovora. A collection of 40 genetically diverse strains of E. amylovora and 55 geographically diverse bacteria that are closely related or share the same ecological niche as E. amylovora were used to test the seven PCR protocols. All bacteria were tested for virulence by inoculation of immature pear fruit and for cultural characteristics on selective media. Only one PCR protocol, Taylor et al. (2001), was specific for all strains of E. amylovora and was able to differentiate E. amylovora from all other bacteria tested. Diagnostic laboratories may need to review their testing procedures in light of these findings.

引用

页码：87 / 97

页数：11

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