A downy mildew effector evades recognition by polymorphism of expression and subcellular localization

被引：35

作者：

Asai, Shuta ^{[1
,2
]}

Furzer, Oliver J. ^{[2
,4
]}

Cevik, Volkan ^{[2
,5
]}

Kim, Dae Sung ^{[2
,6
]}

Ishaque, Naveed ^{[2
,7
]}

Goritschnig, Sandra ^{[3
]}

Staskawicz, Brian J. ^{[3
]}

Shirasu, Ken ^{[1
]}

Jones, Jonathan D. G. ^{[2
]}

机构：

[1] RIKEN, Ctr Sustainable Resource Sci, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan

[2] Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[3] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[4] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

[5] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

[6] Wuhan Univ, Coll Life Sci, Dept Plant Sci, Wuhan 430072, Peoples R China

[7] DKFZ, DKFZ HIPO, Heidelberg Ctr Personalized Oncol, D-69120 Heidelberg, Germany

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

HYALOPERONOSPORA-ARABIDOPSIDIS; GENOME EVOLUTION; RESISTANCE GENES; CELL-DEATH; COMPLEX; PATHOGENS; PROTEINS; PLANTS; EDS1;

D O I：

10.1038/s41467-018-07469-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pathogen co-evolution with plants involves selection for evasion of host surveillance systems. The oomycete Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on Arabidopsis, and race-specific interactions between Arabidopsis accessions and Hpa isolates fit the genefor-gene model in which host resistance or susceptibility are determined by matching pairs of plant Resistance (R) genes and pathogen Avirulence (AVR) genes. Arabidopsis Col-0 carries R gene RPP4 that confers resistance to Hpa isolates Emoy2 and Emwa1, but its cognate recognized effector(s) were unknown. We report here the identification of the Emoy2 AVR effector gene recognized by RPP4 and show resistance-breaking isolates of Hpa on RPP4-containing Arabidopsis carry the alleles that either are not expressed, or show cytoplasmic instead of nuclear subcellular localization.

引用

页数：11

共 52 条

[1] Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis [J].

Aarts, N ;

Metz, M ;

Holub, E ;

Staskawicz, BJ ;

Daniels, MJ ;

Parker, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10306-10311

[2] Natural variation reveals key amino acids in a downy mildew effector that alters recognition specificity by an Arabidopsis resistance gene [J].

Allen, Rebecca L. ;

Meitz, Julia C. ;

Baumber, Rachel E. ;

Hall, Sharon A. ;

Lee, Sarah C. ;

Rose, Laura E. ;

Beynon, Jim L. .

MOLECULAR PLANT PATHOLOGY, 2008, 9 (04) :511-523

[3] Host-parasite coevolutionary conflict between Arabidopsis and downy mildew [J].

Allen, RL ;

Bittner-Eddy, PD ;

Grenvitte-Briggs, LJ ;

Meitz, JC ;

Rehmany, AP ;

Rose, LE ;

Beynon, JL .

SCIENCE, 2004, 306 (5703) :1957-1960

[4] Recent Progress in RXLR Effector Research [J].

Anderson, Ryan G. ;

Deb, Devdutta ;

Fedkenheuer, Kevin ;

McDowell, John M. .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2015, 28 (10) :1063-1072

[5]

Asai S., 2015, BIO-PROTOCOL, V5, pe1627

[6] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana [J].

Asai, Shuta ;

Ohta, Kohji ;

Yoshioka, Hirofumi .

PLANT CELL, 2008, 20 (05) :1390-1406

[7] Expression Profiling during Arabidopsis/Downy Mildew Interaction Reveals a Highly-Expressed Effector That Attenuates Responses to Salicylic Acid [J].

Asai, Shuta ;

Rallapalli, Ghanasyam ;

Piquerez, Sophie J. M. ;

Caillaud, Marie-Cecile ;

Furzer, Oliver J. ;

Ishaque, Naveed ;

Wirthmueller, Lennart ;

Fabro, Georgina ;

Shirasu, Ken ;

Jones, Jonathan D. G. .

PLOS PATHOGENS, 2014, 10 (10)

[8] In Planta Effector Competition Assays Detect Hyaloperonospora arabidopsidis Effectors That Contribute to Virulence and Localize to Different Plant Subcellular Compartments [J].

Badel, Jorge Luis ;

Piquerez, Sophie J. M. ;

Greenshields, David ;

Rallapalli, Ghanasyam ;

Fabro, Georgina ;

Ishaque, Naveed ;

Jones, Jonathan D. G. .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2013, 26 (07) :745-757

[9] Molecular Cloning of ATR5Emoy2 from Hyaloperonospora arabidopsidis, an Avirulence Determinant That Triggers RPP5-Mediated Defense in Arabidopsis [J].

Bailey, Kate ;

Cevik, Volkan ;

Holton, Nicholas ;

Byrne-Richardson, Jane ;

Sohn, Kee Hoon ;

Coates, Mary ;

Woods-Toer, Alison ;

Aksoy, H. Murat ;

Hughes, Linda ;

Baxter, Laura ;

Jones, Jonathan D. G. ;

Beynon, Jim ;

Holub, Eric B. ;

Toer, Mahmut .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2011, 24 (07) :827-838

[10] Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses [J].

Bao, Fei ;

Huang, Xiaozhen ;

Zhu, Chipan ;

Zhang, Xiaoyan ;

Li, Xin ;

Yang, Shuhua .

NEW PHYTOLOGIST, 2014, 202 (04) :1320-1334

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