In vivo genotoxicity study of titanium dioxide nanoparticles using comet assay following intratracheal instillation in rats

被引：47

作者：

Naya, Masato ^{[1
]}

Kobayashi, Norihiro ^{[1
]}

Ema, Makoto ^{[1
]}

Kasamoto, Sawako ^{[2
]}

Fukumuro, Masahito ^{[2
]}

Takami, Shigeaki ^{[2
]}

Nakajima, Madoka ^{[2
]}

Hayashi, Makoto ^{[2
]}

Nakanishi, Junko ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Inst Sci Safety & Sustainabil, Tsukuba, Ibaraki 3058569, Japan

[2] Biosafety Res Ctr Foods Drugs & Pesticides BSRC, Iwata, Shizuoka 4371213, Japan

来源：

REGULATORY TOXICOLOGY AND PHARMACOLOGY | 2012年 / 62卷 / 01期

关键词：

Titanium dioxide; Nanomaterials; Genotoxicity; In vivo; COMPARATIVE PULMONARY TOXICITY; ULTRAFINE TIO2 PARTICLES; EPITHELIAL-CELLS; DNA-DAMAGE; ZINC-OXIDE; FISH CELLS; INHALATION; RESPONSES; PHOTOGENOTOXICITY; PHOTOACTIVATION;

D O I：

10.1016/j.yrtph.2011.12.002

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Titanium dioxide (TiO2) is widely used as a white pigment in paints, plastics, inks, paper, creams, cosmetics, drugs and foods. In the present study, the genotoxicity of anatase TiO2 nanoparticles was evaluated in vivo using the comet assay after a single or repeated intratracheal instillation in rats. The nanoparticles were instilled intratracheally at a dosage of 1.0 or 5.0 mg/kg body weight (single instillation group) and 0.2 or 1.0 mg/kg body weight once a week for 5 weeks (repeated instillation group) into male Sprague-Dawley rats. A positive control, ethyl methanesulfonate (EMS) at 500 mg/kg, was administered orally 3 h prior to dissection. Histopathologically, macrophages and neutrophils were detected in the alveolus of the lung in the 1.0 and 5.0 mg/kg TiO2 groups. In the comet assay, there was no increase in % tail DNA in any of the TiO2 groups. In the EMS group, there was a significant increase in % tail DNA compared with the negative control group. TiO2 nanoparticles in the anatase crystal phase are not genotoxic following intratracheal instillation in rats. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1 / 6

页数：6

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