A role for LEDGF/p75 in targeting HIV DNA integration

被引：505

作者：

Ciuffi, A

Llano, M

Poeschla, E

Hoffmann, C

Leipzig, J

Shinn, P

Ecker, JR

Bushman, F

机构：

[1] Mayo Clin & Mayo Fdn, Coll Med, Program Mol Med, Rochester, MN 55905 USA

[2] Univ Penn, Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA

[3] Salk Inst Biol Studies, Genom Anal Lab, La Jolla, CA 92037 USA

来源：

NATURE MEDICINE | 2005年 / 11卷 / 12期

关键词：

D O I：

10.1038/nm1329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.

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收藏

页码：1287 / 1289

页数：3

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