Expression of membrane-associated proteins within single emulsion cell facsimiles

被引：12

作者：

Chanasakulniyom, Mayuree ^{[1
]}

Martino, Chiara ^{[1
]}

Paterson, David ^{[1
]}

Horsfall, Louise ^{[2
]}

Rosser, Susan ^{[2
]}

Cooper, Jonathan M. ^{[1
]}

机构：

[1] Univ Glasgow, Div Biomed Engn, Sch Engn, Glasgow G12 8LT, Lanark, Scotland

[2] Univ Glasgow, Inst Mol Cell & Syst Biol, Coll Med & Vet & Life Sci, Glasgow G12 8LT, Lanark, Scotland

来源：

ANALYST | 2012年 / 137卷 / 13期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

MONODISPERSE DOUBLE EMULSIONS; MICROFLUIDICS;

D O I：

10.1039/c2an35047e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

MreB is a structural membrane-associated protein which is one of the key components of the bacterial cytoskeleton. Although it plays an important role in shape maintenance of rod-like bacteria, the understanding of its mechanism of action is still not fully understood. This study shows how segmented flow and microdroplet technology can be used as a new tool for biological in vitro investigation of this protein. In this paper, we demonstrate cell-free expression in a single emulsion system to express red fluorescence protein (RFP) and MreB linked RFP (MreB-RFP). We follow the aggregation and localisation of the fusion protein MreB-RFP in this artificial cell-like environment. The expression of MreB-RFP in single emulsion droplets leads to the formation of micrometer-scale protein patches distributed at the water/oil interface.

引用

页码：2939 / 2943

页数：5

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