Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore

被引：99
作者：
Chalmers, Susan [1 ]
Caldwell, Stuart T. [2 ]
Quin, Caroline [2 ]
Prime, Tracy A. [3 ]
James, Andrew M. [3 ]
Cairns, Andrew G. [2 ]
Murphy, Michael P. [3 ]
McCarron, John G. [1 ]
Hartley, Richard C. [2 ]
机构：
[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0RE, Lanark, Scotland
[2] Univ Glasgow, WestCHEM Sch Chem, Glasgow G12 8QQ, Lanark, Scotland
[3] MRC Mitochondrial Biol Unit, Cambridge CB2 0XY, England
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 02期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
HYDROGEN-PEROXIDE; HEART-CELLS; CA2+; HETEROGENEITY; ANTIOXIDANT; DERIVATIVES; MOLECULES; DYNAMICS; RELEASE; ACIDS;
D O I：
10.1021/ja2077922
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predeter mined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
引用
收藏
页码：758 / 761
页数：4
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