Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas

被引：124

作者：

Wang, Yue J. ^{[1
,2
]}

Golson, Maria L. ^{[1
,2
]}

Schug, Jonathan ^{[1
,2
]}

Traum, Daniel ^{[3
,4
]}

Liu, Chengyang ^{[5
]}

Vivek, Kumar ^{[6
]}

Dorrell, Craig ^{[7
]}

Naji, Ali ^{[5
]}

Powers, Alvin C. ^{[8
,9
,10
,11
]}

Chang, Kyong-Mi ^{[3
,4
]}

Grompe, Markus ^{[7
]}

Kaestner, Klaus H. ^{[1
,2
]}

机构：

[1] Univ Penn, Perelman Sch Med, Dept Genet, Philadelphia, PA 19104 USA

[2] Univ Penn, Perelman Sch Med, Inst Diabet Obes & Metab, Philadelphia, PA 19104 USA

[3] Univ Penn, Corporal Michael J Crescenz Vet Affairs Med Ctr, Med Res, Philadelphia, PA 19104 USA

[4] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA

[5] Univ Penn, Perelman Sch Med, Dept Surg, Philadelphia, PA 19104 USA

[6] Montefiore Med Ctr, Albert Einstein Coll Med, Bronx, NY 10467 USA

[7] Oregon Hlth & Sci Univ, Oregon Stem Cell Ctr, Pape Family Pediat Res Inst, Portland, OR 97239 USA

[8] Vanderbilt Univ, Sch Med, Dept Mol Physiol, Nashville, TN USA

[9] Vanderbilt Univ, Sch Med, Dept Biophys, Nashville, TN USA

[10] Vanderbilt Univ, Sch Med, Dept Med, Div Diabet Endocrinol & Metab, Nashville, TN USA

[11] Tennessee Valley Healthcare Syst, Dept Vet Affairs, Nashville, TN 37212 USA

来源：

CELL METABOLISM | 2016年 / 24卷 / 04期

关键词：

PROGENITOR CELLS; FLOW-CYTOMETRY; BETA-CELLS; PROLIFERATION; ALPHA; ADULT; POPULATIONS; TURNOVER; ISLETS; IDENTIFICATION;

D O I：

10.1016/j.cmet.2016.09.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High-dimensional analysis of our marker protein expression revealed three major clusters of beta cells within individuals. Proliferating beta cells are confined to two of the clusters.

引用

页码：616 / 626

页数：11

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