Dielectrophoretic separation of platelets from diluted whole blood in microfluidic channels

被引：158

作者：

Pommer, Matthew S. ^{[2
]}

Zhang, Yanting ^{[2
]}

Keerthi, Nawarathna ^{[2
]}

Chen, Dong ^{[3
]}

Thomson, James A. ^{[4
]}

Meinhart, Carl D. ^{[2
]}

Soh, Hyongsok T. ^{[1
,2
]}

机构：

[1] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Mech Engn, Santa Barbara, CA 93106 USA

[3] Mayo Clin, Div Hematopathol, Rochester, MN USA

[4] Univ Wisconsin, Dept Anat, Madison, WI 53706 USA

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 06期

关键词：

bioseparations; cell sorting; dielectrophoresis; microfluidics; platelets;

D O I：

10.1002/elps.200700607

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The dielectrophoresis (DEP) phenomenon is used to separate platelets directly from diluted whole blood in microfluidic channels. By exploiting the fact that platelets are the smallest cell type in blood, we utilize the DEP-activated cell sorter (DACS) device to perform size-based fractionation of blood samples and continuously enrich the platelets in a label-free manner. Cytometry analysis revealed that a single pass through the two-stage DACS device yields a high purity of platelets (similar to 95%) at a throughput of similar to 2.2 x 10(4) cells/second/microchannel with minimal platelet activation. This work demonstrates gentle and label-free dielectrophoretic separation of delicate cells from complex samples and such a separation approach may open a path toward continuous screening of blood products by integrated microfluidic devices.

引用

页码：1213 / 1218

页数：6

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