Molecular cloning, sequencing, and phylogenetic relationships of a new potyvirus: Sugarcane streak mosaic virus, and a reevaluation of the classification of the Potyviridae

被引:70
作者
Hall, JS
Adams, B
Parsons, TJ
French, R
Lane, LC
Jensen, SG
机构
[1] Univ Nebraska, USDA, ARS, Lincoln, NE 68583 USA
[2] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA
关键词
D O I
10.1006/mpev.1998.0535
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nucleic acid of a serologically distinct potyvirus, originally isolated out of sugar cane from Pakistan, was reverse transcribed and the 3' terminal 2000 bp was PCR amplified, cloned, and sequenced. Phylogenetic comparisons of viruses representing each genus of the Potyviridae show that the Pakistani isolate is most closely related to the rymoviruses wheat streak mosaic virus (WSMV) and brome streak mosaic virus. We therefore propose that this new virus species be named sugar cane streak mosaic virus to reflect its similarity to WSMV. The phylogenetic data also show that the genus Rymovirus contains at least two unique evolutionary lineages. Thus the current taxonomy, based on transmission vector, is paraphyletic. We present an analysis of the taxonomic relationships among members of the family and propose a classification that both resolves the paraphyly and more accurately represents the evolutionary history of the Potyviridae. (C) 1998 Academic Press.
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页码:323 / 332
页数:10
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