Tumor associated antigen and interleukin-12 mRNA transfected dendritic cells enhance effector function of natural killer cells and antigen specific T-cells

被引：56

作者：

Bontkes, Hetty J. ^{[1
]}

Kramer, Duco ^{[1
]}

Ruizendaal, Janneke J. ^{[1
]}

Meijer, Chris J. L. M. ^{[1
]}

Hooijberg, Erik ^{[1
]}

机构：

[1] Vrije Univ Amsterdam Med Ctr, Dept Pathol, Amsterdam, Netherlands

来源：

CLINICAL IMMUNOLOGY | 2008年 / 127卷 / 03期

关键词：

human; dendritic cells; natural killer cells; cytokines; vaccination;

D O I：

10.1016/j.clim.2008.02.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immunotherapy aiming at the combined activation of tumor associated antigen (TAA) specific cytotoxic T lymphocytes (CTL) and Natural Killer (NK) cells may be crucial to eradicate both MHC-I positive and negative tumors. Vaccination with mature dendritic cells (DC) transfected with mRNA encoding for TAA and the pro-inflammatory cytokines interteukin (IL)-12 and IL-18 may increase NK cell and TAA specific CTL activity. We demonstrate here that IL-12 over-expressing human DC induces increased NK cell activation and effector function and confirm the increase in TAA specific CTL by TAA/IL-12 double transfected DC. The effects of IL-18 transfection were limited to phenotypic activation and down-regulation of tissue homing receptors and did not add to the effect of IL-12 on NK cell effector function. In conclusion, co-transfection of TAA and IL-12 mRNA into mature DCs offers a vaccine for the induction of an antitumor immune response mediated by CTL and NK effector cells. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：375 / 384

页数：10

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