Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells

被引：53

作者：

Nishisaka-Nonaka, Risa ^{[1
]}

Mawatari, Kazuaki ^{[1
]}

Yamamoto, Tomomi ^{[1
]}

Kojima, Mizuki ^{[1
]}

Shimohata, Takaaki ^{[1
]}

Uebanso, Takashi ^{[1
]}

Nakahashi, Mutsumi ^{[2
]}

Emoto, Takahiro ^{[3
]}

Akutagawa, Masatake ^{[3
]}

Kinouchi, Yohsuke ^{[3
]}

Wada, Takahiro ^{[4
]}

Okamoto, Masayuki ^{[4
]}

Ito, Hiroshi ^{[4
]}

Yoshida, Ken-ichi ^{[4
]}

Daidoji, Tomo ^{[5
]}

Nakaya, Takaaki ^{[5
]}

Takahashi, Akira ^{[1
]}

机构：

[1] Tokushima Univ, Inst Biomed Sci, Dept Prevent Environm & Nutr, Grad Sch, Kuramoto Cho 3-18-15, Tokushima, Tokushima 7708503, Japan

[2] Tokushima Univ, Grad Sch Biosci & Bioind, Minamijyousanjima Cho 2-1, Tokushima, Tokushima 7708506, Japan

[3] Tokushima Univ, Grad Sch Sci & Technol, Minamijyousanjima Cho 2-1, Tokushima, Tokushima 7708506, Japan

[4] Nihon Funen Co Ltd, 179-1 Mitsujima Shinden,Kawashima Cho, Yoshinogawa City, Tokushima 7793394, Japan

[5] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Infect Dis, Kamigyo Ku, 465 Kajii Cho, Kawaramachi Hirokoji, Kyoto 6028566, Japan

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2018年 / 189卷

关键词：

DISINFECTION BY-PRODUCTS; UV; HEMAGGLUTININ; PERFORMANCE; OXIDATION; REPAIR; BETA; LEDS; H5N1;

D O I：

10.1016/j.jphotobiol.2018.10.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock, especially poultry and pigs. This study aimed to investigate how to inactivate IAVs by using different ultraviolet-light-emitting diodes (UV-LEDs). We developed sterilization equipment with light-emitting diodes (LEDs) those peak wavelengths were 365 nm (UVA-LED), 310 nm (UVB-LED), and 280 nm (UVC-LED). These UV-LED irradiations decreased dose fluence-dependent plaque-forming units of IAV H1N1 subtype (A/Puerto Rico/8/1934) infected Madin-Darby canine kidney (MDCK) cells, but the inactivation efficiency of UVA-LED was significantly lower than UVB- and UVC-LED. UV-LED irradiations did not alter hemagglutination titer, but decreased accumulation of intracellular total viral RNA in infected MDCK cells was observed. Additionally, UV-LED irradiations suppressed the accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR. These results suggest that UV-LEDs inhibit host cell replication and transcription of viral RNA. Both UVB- and UVC-LED irradiation decreased focus-forming unit (FFU) of H5N1 subtype (A/Crow/Kyoto/53/2004), a highly pathogenic avian IAV (HPAI), in infected MDCK cells, and the amount of FFU were lower than the H1N1 subtype. From these results, it appears that IAVs may have different sensitivity among the subtypes, and UVB- and UVC-LED may be suitable for HPAI virus inactivation.

引用

页码：193 / 200

页数：8

共 36 条

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