Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV)

被引：21

作者：

Li, Bin ^{[1
,2
,3
,4
]}

Xiao, Shaobo ^{[4
]}

Ma, Junjie ^{[1
,2
,3
]}

Liu, Yanling ^{[1
,2
,3
]}

Mao, Li ^{[1
,2
,3
]}

Wen, Libin ^{[1
,2
,3
]}

Mao, Aihua ^{[1
,2
,3
]}

Zhang, Xuehan ^{[1
,2
,3
]}

Ni, Yanxiu ^{[1
,2
,3
]}

Guo, Rongli ^{[1
,2
,3
]}

Zhou, Junming ^{[1
,2
,3
]}

Yu, Zhengyu ^{[1
,2
,3
]}

Lv, Lixin ^{[1
,2
,3
]}

Wang, Xiaomin ^{[1
,2
,3
]}

Fang, Liurong ^{[4
]}

Chen, Huanchun ^{[4
]}

He, Kongwang ^{[1
,2
,3
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu Prov, Peoples R China

[2] Minist Agr, Key Lab Anim Dis Diagnost & Immunol, Nanjing 210014, Jiangsu Prov, Peoples R China

[3] Natl Ctr Engn Res Vet Bioprod, Nanjing 210014, Jiangsu Prov, Peoples R China

[4] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Div Anim Infect Dis, Wuhan 430070, Peoples R China

来源：

VIROLOGY JOURNAL | 2011年 / 8卷

基金：

国家高技术研究发展计划(863计划);

关键词：

BOVINE PARVOVIRUS; IDENTIFICATION; CLONING;

D O I：

10.1186/1743-422X-8-357

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recently discovered porcine boca-like virus (Pbo-likeV) is a member of the Parvoviridae family, genus Bocavirus, and it is potentially associated with swine disease. Several studies have associated Pbo-likeV with postweaning multisystemic wasting syndrome in pigs, but the full spectrum of clinical disease and the epidemiology of Pbo-likeV infection remain unclear. The availability of rapid and reliable molecular diagnostics would aid future studies of this novel virus. Thus, we developed a sensitive and specific TaqMan-based real-time PCR assay to target the Pbo-likeV NP1 gene. The assay reproducibly detected 20 copies of a recombinant DNA plasmid containing the NP1 gene, with a dynamic range of six orders of magnitude (10(2)-10(7) copies). The assay did not cross-react with other animal viruses. Clinical evaluation found that Pbo-likeV was present in Chinese swine herds at a frequency of 44.2% (114/258). Higher infection rates were found in diseased pigs (56.1%, 101/180) compared with healthy pigs (16.7%, 13/78) (P < 0.05). Our assay for the diagnosis and quantification of Pbo-likeV was highly sensitive and specific, and should provide a reliable real-time tool for epidemiological and pathogenetic study of Pbo-likeV infection.

引用

页数：5

共 15 条

[1] Cloning of a human parvovirus by molecular screening of respiratory tract samples [J].