Tetranucleotide microsatellite markers in Cryptomeria japonica D. Don

被引:7
作者
Guan, L. [2 ]
Shiraishi, S. [1 ]
机构
[1] Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan
[2] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan
关键词
Japanese cedar; Cryptomeria japonica; Tetranucleotide microsatellite; Suppression PCR; LINKAGE MAP;
D O I
10.1007/s12686-010-9342-y
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Japanese cedar (Cryptomeria japonica D. Don) is an important ecological and commercial species in Japan. We isolated 11 polymorphic tetranucleotide microsatellite markers by using a ligation-mediated suppression PCR technique for Japanese cedar and investigated the polymorphism of these markers in 24 individuals from natural populations. The number of alleles ranged from 6 to 20 per locus, and expected heterozygosities of the 11 loci ranged from 0.664 to 0.950. These polymorphic markers will be powerful tools for the studies of individual genotyping, population genetics, and conservation genetics.
引用
收藏
页码:283 / 285
页数:3
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