The relation between IL-10 gene (-1082G/A) and VEGF gene 936 C/T polymorphism and diabetic polyneuropathy in a cohort of Egyptian patients with type 2 diabetes

Background. Polymorphisms have been described to be correlated with type 2 diabetes mellitus (T2DM) and its complications including diabetic polyneuropathy (DPN). The aim of this study was to investigate the relation between interleukin (IL)-10-1082 G/A (rs1800896) and vascular endothelial growth factor (VEGF)-936 C/T polymorphisms and DPN in T2DM patients. Methods. This cross-sectional study included 50 T2DM patients and 40 controls. Clinical and electrophysiological assessments for DPN, fasting blood glucose level (FBS) and glycosylated hemoglobin (HbA1 C) were recorded. VEGF-936 C/T polymorphism was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping for IL-10 promotor gene (-1082 G/A) (rs1800896) polymorphism was performed using Real-time PCR. Results. Sixty percent of patients had confirmed DPN, while none of them were considered normal. IL-10 (genotype -1082G/G) was statistically higher among controls compared to T2DM patients (P = 0.008). VEGF-936-CT genotype was statistically higher among patients compared to controls (P = 0.033), but there was no significant relation between IL-10-1082 G/A or VEGF-936 C/T polymorphism genotypes and DPN. Patients with IL-10 (genotype-1082A/G) had higher H bA1c level (P = 0.041) and lower albumin/creatinine ratio, while those with IL-10 (genotype-1082G/G) had higher albumin/creatinine ratio (P = 0.024). Thus, DPN has significant correlation with the duration of diabetes, FBS and HbA1c. Conclusion: The VEGF-936 C/T genotype may be associated with T2DM in contrast to IL-10 (genotype1082G/G) which may be less likely to be associated with it. However, there is no association between VEGF-936 or IL-10-1082 genotypes and DPN.