Serotyping of Toxoplasma gondii:: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

被引：57

作者：

Morisset, Stephane ^{[2
]}

Peyron, Francois ^{[1
,3
]}

Lobry, Jean R. ^{[2
]}

Garweg, Justus ^{[4
]}

Ferrandiz, Josette ^{[3
]}

Musset, Karine ^{[9
,10
]}

Gomez-Marin, Jorge Enrique ^{[5
]}

de la Torre, Alejandra ^{[5
]}

Demar, Magali ^{[6
,7
]}

Carme, Bernard ^{[8
]}

Mercier, Corinne ^{[9
,10
]}

Garin, Jean Francois ^{[11
]}

Cesbron-Delauw, Marie-France ^{[9
,10
]}

机构：

[1] Hosp Civils Lyon, Hop Croix Rousse, Serv Parasitol & Pathol Exot, Lyon, France

[2] Univ Lyon 1, CNRS, Lab Biometrie & Biol Evolut UMR 5558, F-69622 Villeurbanne, France

[3] Fac Med Lyon 1, Lab Parasitol & Pathol Exot, Lyon, France

[4] Swiss Eye Inst, Clin Inflammatory Eye Dis, Bern, Switzerland

[5] Univ Quindio, Ctr Invest Biomed, GEPAMOL, Armenia, Colombia

[6] Trop Hyg Hosp, Unite Malad Infect, Cayenne 97306, French Guiana

[7] Trop Hyg Hosp, Lab Parasitol Mycol, Equipe EA 3593, Ctr Hosp Andree Rosemon, Cayenne 97306, French Guiana

[8] Epidemiol Parasitoses Trop UAG, Equipe EA 3593, Cayenne 97306, French Guiana

[9] Univ Grenoble 1, Lab Adaptat & Pathogenie Micro Organismes, Grenoble 1, France

[10] CNRS UMR 5163, Grenoble, France

[11] Univ Paris 07, Fac Med, Lab Parasitol Mycol, Paris, France

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 07期

关键词：

Toxoplasma gondii isolates; serotyping; Type I; II; III; GRA5; GRA6;

D O I：

10.1016/j.micinf.2008.04.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Field isolates of Toxoplasma gondii in Europe and North America have been grouped into three clonal lineages that display different virulence in mice. Whether the genetic structure of the parasite is related to clinical expression in humans has not yet been demonstrated. We developed an enzyme-linked immunosorbent assay which uses lineage-specific, polymorphic polypeptides derived from the dense granule antigens, GRA5 and GRA6. Our goal was to compare serotypical patterns observed in asymptomatic versus symptomatic (ocular disease and severe infection in human immunodeficiency virus (HIV)-positive patients) infections among patients from Europe and South America. Independent of the clinical presentation of the disease, serotypes differed according to geographical origin, with a homogeneous distribution of serotype 11 in Europe and of serotypes I and III in South America. We conclude that GRA5-GRA6 serotyping is an interesting tool to study serotype prevalence in populations but it is not an accurate marker of pathogenicity of Toxoplasma infection in humans. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：742 / 747

页数：6

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