Trapped in the act of catalysis

被引：11

作者：

Davies, GJ ^{[1
]}

Wilson, KS ^{[1
]}

机构：

[1] Univ York, Dept Chem, Struct Biol Lab, York YO10 5DD, N Yorkshire, England

来源：

NATURE STRUCTURAL BIOLOGY | 1999年 / 6卷 / 05期

关键词：

D O I：

10.1038/8200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of modified substrates, freeze-trapping and site-directed mutagenesis provides an elegant description of catalysis in the ubiquitous a-amylase family.

引用

页码：406 / 408

页数：3

共 11 条

[1] Snapshots along an enzymatic reaction coordinate:: Analysis of a retaining β-glycoside hydrolase [J].

Davies, GJ ;

Mackenzie, L ;

Varrot, A ;

Dauter, M ;

Brzozowski, AM ;

Schülein, M ;

Withers, SG .

BIOCHEMISTRY, 1998, 37 (34) :11707-11713

[2] Structural and sequence-based classification of glycoside hydrolases [J].

Henrissat, B ;

Davies, G .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (05) :637-644

[3] Updating the sequence-based classification of glycosyl hydrolases [J].

Henrissat, B ;

Bairoch, A .

BIOCHEMICAL JOURNAL, 1996, 316 :695-696

[4] GLYCOSYLATION INHIBITORS IN BIOLOGY AND MEDICINE [J].

JACOB, GS .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (05) :605-611

[5] The early history of lysozyme [J].

Johnson, LN .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (11) :942-944

[6] STEREOCHEMISTRY AND THE MECHANISM OF ENZYMATIC REACTIONS [J].

KOSHLAND, DE .

BIOLOGICAL REVIEWS OF THE CAMBRIDGE PHILOSOPHICAL SOCIETY, 1953, 28 (04) :416-436

[7] Insights into transition state stabilization of the β-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants [J].

Notenboom, V ;

Birsan, C ;

Nitz, M ;

Rose, DR ;

Warren, RAJ ;

Withers, SG .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (09) :812-818

[8] INACTIVATION OF A BETA-GLUCOSIDASE THROUGH THE ACCUMULATION OF A STABLE 2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSYL ENZYME INTERMEDIATE - A DETAILED INVESTIGATION [J].

STREET, IP ;

KEMPTON, JB ;

WITHERS, SG .

BIOCHEMISTRY, 1992, 31 (41) :9970-9978

[9] Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity [J].

Strokopytov, B ;

Knegtel, RMA ;

Penninga, D ;

Rozeboom, HJ ;

Kalk, KH ;

Dijkhuizen, L ;

Dijkstra, BW .

BIOCHEMISTRY, 1996, 35 (13) :4241-4249

[10] X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the α-amylase family [J].

Uitdehaag, JCM ;

Mosi, R ;

Kalk, KH ;

van der Veen, BA ;

Dijkhuizen, L ;

Withers, SG ;

Dijkstra, BW .

NATURE STRUCTURAL BIOLOGY, 1999, 6 (05) :432-436

← 1 2 →