A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection

被引：4

作者：

Marrakchi, M. ^{[1
]}

Campos Sanchez, I. ^{[2
]}

Helali, S. ^{[3
]}

Mejri, N. ^{[1
]}

Soto Camino, J. ^{[2
]}

Gonzalez-Martinez, M. A. ^{[2
]}

Hamdi, M. ^{[1
]}

Abdelghani, A. ^{[3
]}

机构：

[1] Carthage Univ, Lab Ecol & Technol Microbienne, INSAT, Charguia 1080, Tunisia

[2] Univ Politecn Valencia, Dept Quim, Valencia 46022, Spain

[3] Carthage Univ, Nanotechnol Lab, INSAT, Charguia 1080, Tunisia

来源：

SENSOR LETTERS | 2011年 / 9卷 / 06期

关键词：

Immunosensor; Atrazine; Physisorption; Electrochemical Impedance Spectroscopy; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; ATRAZINE RESIDUES; SAMPLES; IMMUNOASSAY; PERFORMANCE; HERBICIDES; DESIGN; WATER; DISKS;

D O I：

10.1166/sl.2011.1800

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive, simple and label-free immunosensor for atrazine detection was developed. The immunosensor was based on antibody physisorption as immobilization technique. This technique was proposed as a cost-effective gold immuno-functionalization strategy alternative to SAM-based strategy for pesticide detection. Each step of immunosensor construction was characterized by electrochemical impedance spectroscopy, and the antibody-atrazine interaction was measured in a dynamic range from 10 to 150 ng/mL. This method was highly selective for atrazine and propazine, showing weak or no cross-reactivity to other similar compounds like terbutylazine. A limit detection of 10 ng/mL of atrazine was detected in PBS buffer with a good reproductibility. Lower limit detection in tap water was obtained.

引用

页码：2203 / 2206

页数：4

共 17 条

[1] USE OF EXTRACTION DISKS FOR TRACE ENRICHMENT OF VARIOUS PESTICIDES FROM RIVER WATER AND SIMULATED SEAWATER SAMPLES FOLLOWED BY LIQUID-CHROMATOGRAPHY RAPID-SCANNING UV VISIBLE AND THERMOSPRAY MASS-SPECTROMETRY DETECTION [J].

BARCELO, D ;

DURAND, G ;

BOUVOT, V ;

NIELEN, M .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (02) :271-277

[2] A Physisorbed Interface Design of Biomolecules for Selective and Sensitive Protein Detection [J].

Choi, Seokheun ;

Chae, Junseok .

JALA, 2010, 15 (03) :172-178

[3] In situ electrodepo sited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design [J].

Du, Dan ;

Ding, Jiawang ;

Cai, Jie ;

Zhang, Jianming ;

Liu, Li .

TALANTA, 2008, 74 (05) :1337-1343

[4]

EEC Directive, 1980, OFFICIAL J EUROPEAN

[5] Rapid immunoanalytical method for the determination of atrazine residues in olive oil [J].

Garcés-García, M ;

Morais, S ;

Gonzaález-Martínez, MA ;

Puchades, R ;

Maquieira, A .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 378 (02) :484-489

[6] Development of a highly sensitive enzyme-linked immunosorbent assay for atrazine - Performance evaluation by flow injection immunoassay [J].

Gascon, J ;

Oubina, A ;

Ballesteros, B ;

Barcelo, D ;

Camps, F ;

Marco, MP ;

GonzalezMartinez, MA ;

Morais, S ;

Puchades, R ;

Maquieira, A .

ANALYTICA CHIMICA ACTA, 1997, 347 (1-2) :149-162

[7]

Gascon J., 1997, ACS SYM SER, V657, P254

[8]

González-Martínez MA, 1999, ANAL CHIM ACTA, V386, P201, DOI 10.1016/S0003-2670(99)00032-X

[9] Atrazine analysis using an impedimetric immunosensor based on mixed biotinylated self-assembled monolayer [J].

Hleli, S ;

Martelet, C ;

Abdelghani, A ;

Burais, N ;

Jaffrezic-Renault, N .

SENSORS AND ACTUATORS B-CHEMICAL, 2006, 113 (02) :711-717

[10] Breakthrough volumes increased by the addition of salt in the on-line solid-phase extraction and liquid chromatography of pesticides in environmental water [J].

MartinEsteban, A ;

Fernandez, P ;

Camara, C .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1996, 63 (02) :127-135

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