E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming

被引:246
作者
Redmer, Torben [1 ,2 ]
Diecke, Sebastian [1 ,2 ]
Grigoryan, Tamara [1 ,3 ]
Quiroga-Negreira, Angel [1 ,3 ]
Birchmeier, Walter [1 ]
Besser, Daniel [1 ]
机构
[1] Max Delbrueck Ctr, D-13125 Berlin, Germany
[2] Free Univ Berlin, Dept Biol Chem & Pharm, D-14195 Berlin, Germany
[3] Humboldt Univ, Fac Math & Nat Sci 1, D-10099 Berlin, Germany
关键词
pluripotency; somatic cell reprogramming; E-cadherin; OCT4; SELF-RENEWAL; CONTACT;
D O I
10.1038/embor.2011.88
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report new functions of the cell-adhesion molecule E-cadherin in murine pluripotent cells. E-cadherin is highly expressed in mouse embryonic stem cells, and interference with E-cadherin causes differentiation. During cellular reprogramming of mouse fibroblasts by OCT4, SOX2, KLF4 and c-MYC, fully reprogrammed cells were exclusively observed in the E-cadherinpositive cell population and could not be obtained in the absence of E-cadherin. Moreover, reprogrammed cells could be established by viral E-cadherin in the absence of exogenous OCT4. Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming.
引用
收藏
页码:720 / 726
页数:7
相关论文
共 20 条
[1]   E-cadherin is a survival factor for the lactating mouse mammary gland [J].
Boussadia, O ;
Kutsch, S ;
Hierholzer, A ;
Delmas, V ;
Kemler, R .
MECHANISMS OF DEVELOPMENT, 2002, 115 (1-2) :53-62
[2]   E-Cadherin-Mediated Cell-Cell Contact Is Critical for Induced Pluripotent Stem Cell Generation [J].
Chen, Taotao ;
Yuan, Detian ;
Wei, Bin ;
Jiang, Jing ;
Kang, Jiuhong ;
Ling, Kun ;
Gu, Yijun ;
Li, Jinsong ;
Xiao, Lei ;
Pei, Gang .
STEM CELLS, 2010, 28 (08) :1315-1325
[3]   The Growth Factor Environment Defines Distinct Pluripotent Ground States in Novel Blastocyst-Derived Stem Cells [J].
Chou, Yu-Fen ;
Chen, Hsu-Hsin ;
Eijpe, Maureen ;
Yabuuchi, Akiko ;
Chenoweth, Joshua G. ;
Tesar, Paul ;
Lu, Jun ;
Mckay, Ronald D. G. ;
Geijsen, Niels .
CELL, 2008, 135 (03) :449-461
[4]   FGF2 signaling in mouse embryonic fibroblasts is crucial for self-renewal of embryonic stem cells [J].
Diecke, Sebastian ;
Quiroga-Negreira, Angel ;
Redmer, Torben ;
Besser, Daniel .
CELLS TISSUES ORGANS, 2008, 188 (1-2) :52-61
[5]   Rho-family GTPases in cadherin-mediated cell-cell adhesion [J].
Fukata, M ;
Kaibuchi, K .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (12) :887-897
[6]   Interplay of Cadherin-Mediated Cell Adhesion and Canonical Wnt Signaling [J].
Heuberger, Julian ;
Birchmeier, Walter .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2010, 2 (02) :a002915
[7]   Epigenetic reprogramming and induced pluripotency [J].
Hochedlinger, Konrad ;
Plath, Kathrin .
DEVELOPMENT, 2009, 136 (04) :509-523
[8]   Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming [J].
Jaenisch, Rudolf ;
Young, Richard .
CELL, 2008, 132 (04) :567-582
[9]   Embryonic stem cell-specific microRNAs promote induced pluripotency [J].
Judson, Robert L. ;
Babiarz, Joshua E. ;
Venere, Monica ;
Blelloch, Robert .
NATURE BIOTECHNOLOGY, 2009, 27 (05) :459-461
[10]   β-Catenin Enhances Oct-4 Activity and Reinforces Pluripotency through a TCF-Independent Mechanism [J].
Kelly, Kevin F. ;
Ng, Deborah Y. ;
Jayakumaran, Gowtham ;
Wood, Geoffrey A. ;
Koide, Hiroshi ;
Doble, Bradley W. .
CELL STEM CELL, 2011, 8 (02) :214-227