Carbonic Anhydrase III Promotes Cell Migration and Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma

被引：18
作者：
Chu, Yin-Hung [1 ]
Su, Chun-Wen [1 ,2 ]
Hsieh, Yih-Shou [3 ]
Chen, Pei-Ni [3 ]
Lin, Chiao-Wen [4 ,5 ]
Yang, Shun-Fa [1 ,2 ]
机构：
[1] Chung Shan Med Univ, Inst Med, Taichung 402, Taiwan
[2] Chung Shan Med Univ Hosp, Dept Med Res, Taichung 402, Taiwan
[3] Chung Shan Med Univ, Inst Biochem Microbiol & Immunol, Taichung 402, Taiwan
[4] Chung Shan Med Univ, Inst Oral Sci, Taichung 402, Taiwan
[5] Chung Shan Med Univ Hosp, Dept Dent, Taichung 402, Taiwan
来源：
CELLS | 2020年 / 9卷 / 03期
关键词：
CA III; epithelial-mesenchymal transition; oral cancer; metastasis; EPIGENETIC CONTROL; NECK-CANCER; E-CADHERIN; METASTASIS; HEAD; EXPRESSION; INVASION; EMT; TRANSFORMATION; FIBRONECTIN;
D O I：
10.3390/cells9030704
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Epithelial-mesenchymal transition (EMT) is strongly correlated with tumor metastasis and contains several protein markers, such as E-cadherin. Carbonic anhydrase III (CA III) exhibits low carbon dioxide hydratase activity in cancer. However, the detailed mechanisms of CA III and their roles in oral cancer are still unknown. This study established a CA III-overexpressed stable clone and observed the expression of CA III protein in human SCC-9 and SAS oral cancer cell lines. The migration and invasion abilities were determined using a Boyden chamber assay. Our results showed that the overexpression of CA III protein significantly increased the migration and invasion abilities in oral cancer cells. Moreover, a whole genome array analysis revealed that CA III regulated epithelial-mesenchymal transition by reducing the expression of epithelial markers. Data from the GEO database also demonstrated that CA III mRNA is negatively correlated with CDH1 mRNA. Mechanistically, CA III increased the cell motility of oral cancer cells through the FAK/Src signaling pathway. In conclusion, this suggests that CA III promotes EMT and cell migration and is potentially related to the FAK/Src signaling pathway in oral cancer.
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页数：12
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