Anti-adenovirus Activities of Shikonin, a Component of Chinese Herbal Medicine in Vitro

被引:41
作者
Gao, Hong [1 ]
Liu, Lei [1 ,2 ]
Qu, Zhang-yi [1 ]
Wei, Feng-xiang [1 ,3 ]
Wang, Shu-qiu [2 ]
Chen, Guang [2 ]
Qin, Le [1 ]
Jiang, Fu-yang [1 ]
Wang, Ying-chen [1 ]
Shang, Lei [1 ]
Gao, Chun-yan [4 ]
机构
[1] Harbin Med Coll, Coll Publ Hlth, Dept Hyg Microbiol, Harbin 150081, Peoples R China
[2] Jamusi Univ, Coll Basic Med Sci, Jamusi 154007, Peoples R China
[3] Guangdong Pharmaceut Univ, Dept Biol, Guangzhou 510006, Guangdong, Peoples R China
[4] Harbin Med Coll, Daqing Branch, Daqing 163319, Peoples R China
基金
中国国家自然科学基金; 黑龙江省自然科学基金;
关键词
anti-adenovirus type 3; shikonin; in vitro; HOSPITALIZED CHILDREN; INFECTION; APOPTOSIS; GROWTH; DERIVATIVES; INHIBITION; INDUCTION; PATHWAY; PROTEIN; HEXON;
D O I
10.1248/bpb.34.197
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Radix Lithosperm eyrthrorhizon is a common prescription compound in traditional Chinese medicine. Shikonin is a major component of Radix Lithospermi and has various biological activities. We have investigated the inhibitory effect of shikonin on the growth of adenovirus type 3 (AdV3) in vitro. The antiviral function of shikonin against AdV3 and its virus inhibition ratio were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide method (MTT). The expression of hexon protein in AdV3 was determined by immunofluorescence assay using laser scanning confocal microscopy (LSCM) and Western blot analysis. In addition, the rate of apoptosis in cells infected by AdV3 was determined by flow cytometry. Shikonin (0.0156-1 mu M) inhibited growth of AdV3 in a concentration-dependent manner with a virus inhibition rate of 23.8-69.1%. Expression of hexon protein in AdV3 was higher in the virus control group than in the shikonin-treated groups as determined by immunofluorescence assay and Western blotting (p<0.05). The rate of shikonin-treated HeLa cell apoptosis had a statistically significant decrease with increasing concentration of drug (p<0.05). Our data demonstrate that shikonin possesses anti-AdV3 capabilities and that the potential antiviral mechanism might involve inhibiting the degree of apoptosis and hexon protein expression of AdV.
引用
收藏
页码:197 / 202
页数:6
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