Liquid-liquid equilibrium of poly (ethylene glycol) 4000+sodium sulfate plus urea/guanidine hydrochloride aqueous two-phase systems at different pH: Experimental results and thermodynamic modeling

被引：13

作者：

Pirdashti, Mohsen ^{[1
]}

Movagharnejad, Kamyar ^{[1
]}

Mobalegholeslam, Poorya ^{[2
]}

Pirdashti, Hemmatollah ^{[3
]}

机构：

[1] Babol Univ Technol, Fac Chem Engn, POB 484, Babol Sar, Iran

[2] Islamic Azad Univ, Mahshahr Branch, Dept Chem Engn, Mahshahr, Iran

[3] Sari Agr Sci & Nat Resources Univ, Dept Agron, Genet & Agr Biotechnol Inst Tabarestan, Sari, Iran

来源：

FLUID PHASE EQUILIBRIA | 2016年 / 427卷

关键词：

Aqueous two-phase systems; Phase diagram; Denaturants; Partition coefficient; Extended UNIQUAC model; Modified UNIQUAC-FV; LOCAL COMPOSITION MODEL; EXCESS GIBBS ENERGY; ACTIVITY-COEFFICIENTS; POLYETHYLENE-GLYCOL; WILSON MODEL; EXPRESSION; EXTENSION; K2HPO4; WATER; UREA;

D O I：

10.1016/j.fluid.2016.07.032

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

Aqueous two-phase systems in the presence of denaturants (urea or guanidine hydrochloride) have been used for the initial recovery steps during protein refolding process. To investigate phase behavior of such systems, liquid-liquid equilibrium data for a poly (ethylene glycol) (PEG) 4000 + sodium sulfate + urea/guanidine hydrochloride (5% and 10% mass) aqueous two phase system was investigated at 25 degrees C and pH values of 5.3 and 9.2. The calibration plots for refractive index and conductivity were prepared to obtain the compositions of the top and bottom phase. It was observed that the heterogeneous region decreases with the increase in the denaturants concentration. The effect of pH, denaturants concentration, tie line length, and slope of tie line on the partition behavior of denaturants was examined. In this study, the modified UNIQUAC-FV and extended UNIQUAC models are used to calculate the phase equilibria of mention aqueous two phase systems. The values of average AAD% (partition coefficients) for modified UNIQUAC-FV and extended UNIQUAC are 3.48 and 13.80, respectively. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：460 / 475

页数：16

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