Utilizing Heterostructured Porous Particles to Improve Traditional Paper Chromatography for Spontaneous Protein Separation

被引：2

作者：

Cheng, Xu ^{[1
]}

Yang, Yuemeng ^{[1
]}

Song, Yongyang ^{[2
,4
]}

Xu, Li-Ping ^{[1
]}

Wang, Shutao ^{[2
,3
]}

机构：

[1] Univ Sci & Technol Beijing, Beijing Key Lab Bioengn & Sensing Technol, Sch Chem & Biol Engn, Beijing 100083, Peoples R China

[2] Chinese Acad Sci, CAS Key Lab Bioinspired Mat & Interfacial Sci, CAS Ctr Excellence Nanosci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

来源：

LANGMUIR | 2022年 / 38卷 / 14期

基金：

中国博士后科学基金; 中国国家自然科学基金; 国家重点研发计划;

关键词：

MEMBRANES;

D O I：

10.1021/acs.langmuir.1c03394

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chromatography is a classical technique for protein A separation. However, the chromatography column is filled with tightly packed separation materials and requires an additional pressurizing pump to propel the flow of fluidic samples, largely restraining their applications. Here, we combine heterostructured porous particles with paper strips, realizing spontaneous separation of similarly sized proteins. The interconnected nanofibrous structure and good hydrophility of paper strips enable the spontaneous flow of the liquid sample, and the heterostructured porous particles provide versatile tools for protein separation via electrostatic interaction. The fabricated paper strips are inexpensive, user-friendly, and disposable and exhibit good separation performance. This work may offer a new avenue for fabricating on-site bioseparation tools and purifying various biomacromolecules.

引用

页码：4250 / 4255

页数：6

共 45 条

[1] Elastocapillarity: When Surface Tension Deforms Elastic Solids [J].

Bico, Jose ;

Reyssat, Etienne ;

Roman, Benoit .

ANNUAL REVIEW OF FLUID MECHANICS, VOL 50, 2018, 50 :629-659

[2] Recent Developments in Paper-Based Microfluidic Devices [J].

Cate, David M. ;

Adkins, Jaclyn A. ;

Mettakoonpitak, Jaruwan ;

Henry, Charles S. .

ANALYTICAL CHEMISTRY, 2015, 87 (01) :19-41

[3] Fast, low-pressure chromatographic separation of proteins using hydroxyapatite nanoparticles [J].

Chen, Guoqiang ;

Zhitomirsky, Igor ;

Ghosh, Raja .

TALANTA, 2019, 199 :472-477

[4] Distance-based carcinoembryonic antigen assay on microfluidic paper immunodevice [J].

Chen, Ying ;

Chu, Weiru ;

Liu, Wei ;

Guo, Xiaoyan .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 260 :452-459

[5] Qualitative analysis of proteins: a partition chromatographic method using paper [J].

Consden, R ;

Gordon, AH ;

Martin, AJP .

BIOCHEMICAL JOURNAL, 1944, 38 :224-232

[6]

de Abreu D.d.S., 2020, Comprehensive Analytical Chemistry, P361, DOI [10.1016/bs.coac.2020.01.002, DOI 10.1016/BS.COAC.2020.01.002]

[7] Review - Mass spectrometry and protein analysis [J].

Domon, B ;

Aebersold, R .

SCIENCE, 2006, 312 (5771) :212-217

[8] The predawn of paper chromatography [J].

Ettre, LS .

CHROMATOGRAPHIA, 2001, 54 (5-6) :409-414

[9] Shapeable, Underwater Superelastic, and Highly Phosphorylated Nanofibrous Aerogels for Large-Capacity and High-Throughput Protein Separation [J].

Fu, Qiuxia ;

Liu, Lifang ;

Si, Yang ;

Yu, Jianyong ;

Ding, Bin .

ACS APPLIED MATERIALS & INTERFACES, 2019, 11 (47) :44874-44885

[10] Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation [J].

Fu, Qiuxia ;

Si, Yang ;

Duan, Cheng ;

Yan, Zishuo ;

Liu, Lifang ;

Yu, Jianyong ;

Ding, Bin .

ADVANCED FUNCTIONAL MATERIALS, 2019, 29 (13)

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