The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

被引：50

作者：

Khalifa, Ahmad Abdelzaher Zaki ^{[1
,2
]}

Ichikawa, Muneyoshi ^{[1
,5
]}

Dai, Daniel ^{[1
,2
]}

Kubo, Shintaroh ^{[2
,3
]}

Black, Corbin Steven ^{[1
,2
]}

Peri, Katya ^{[1
]}

McAlear, Thomas S. ^{[1
,2
]}

Veyron, Simon ^{[2
,4
]}

Yang, Shun Kai ^{[1
,2
]}

Vargas, Javier ^{[1
,2
]}

Bechstedt, Susanne ^{[1
,2
]}

Trempe, Jean-Francois ^{[2
,4
]}

Khanh Huy Bui ^{[1
,2
]}

机构：

[1] McGill Univ, Dept Anat & Cell Biol, Quebec City, PQ, Canada

[2] McGill Univ, Ctr Rech Biol Struct FRQS, Quebec City, PQ, Canada

[3] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto, Japan

[4] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ, Canada

[5] Nara Inst Sci & Technol, Grad Sch Biol Sci, Dept Syst Biol, Nara, Japan

来源：

ELIFE | 2020年 / 9卷

基金：

日本学术振兴会; 加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

DOUBLET MICROTUBULES; DYNEIN ARMS; PROTEIN; PARKIN; ACETYLTRANSFERASE; IDENTIFICATION; ACETYLATION; VISUALIZATION; ARCHITECTURE; ALPHA-TAT1;

D O I：

10.7554/eLife.52760

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the alpha- and beta-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule. Microtubule also exists as highly stable doublet microtubules in the cilia where stability is needed for ciliary beating and function. The doublet microtubule maintains its stability through interactions at its inner and outer junctions where its A-and B-tubules meet. Here, using cryo-electron microscopy, bioinformatics and mass spectrometry of the doublets of Chlamydomonas reinhardtii and Tetrahymena thermophila, we identified two new inner junction proteins, FAP276 and FAP106, and an inner junction-associated protein, FAP126, thus presenting the complete answer to the inner junction identity and localization. Our structural study of the doublets shows that the inner junction serves as an interaction hub that involves tubulin post-translational modifications. These interactions contribute to the stability of the doublet and hence, normal ciliary motility.

引用

页数：25

共 65 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].