Fecal Microbiota and Gut Microbe-Derived Extracellular Vesicles in Colorectal Cancer

被引：66
作者：
Park, Jihye [1 ]
Kim, Nam-Eun [2 ]
Yoon, Hyuk [3 ]
Shin, Cheol Min [3 ]
Kim, Nayoung [3 ]
Lee, Dong Ho [3 ]
Park, Jae Yong [4 ]
Choi, Chang Hwan [4 ]
Kim, Jae Gyu [4 ]
Kim, Yoon-Keun [5 ]
Shin, Tae-Seop [5 ]
Yang, Jinho [5 ]
Park, Young Soo [3 ]
机构：
[1] Yonsei Univ, Dept Internal Med, Coll Med, Seoul, South Korea
[2] Seoul Natl Univ, Grad Sch Publ Hlth, Dept Publ Hlth Sci, Seoul, South Korea
[3] Seoul Natl Univ, Dept Internal Med, Bundang Hosp, Seongnam, South Korea
[4] Chung Ang Univ, Dept Internal Med, Coll Med, Seoul, South Korea
[5] Inst MD Healthcare Inc, R&D Ctr, Seoul, South Korea
来源：
FRONTIERS IN ONCOLOGY | 2021年 / 11卷
基金：
新加坡国家研究基金会;
关键词：
microbiome; metagenome; gut microbe-derived extracellular vesicles; colorectal cancer; cancer stage; DIETARY PATTERNS; EXOSOMES;
D O I：
10.3389/fonc.2021.650026
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The human microbiota comprises trillions of microbes, and the relationship between cancer and microbiota is very complex. The impact of fecal microbiota alterations on colorectal cancer (CRC) pathogenesis is emerging. This study analyzed changes in the microbial composition in CRC subjects with both fecal microbiota and gut microbe-derived extracellular vesicles (EVs). From August 2017 to August 2018, 70 CRC patients and 158 control subjects were enrolled in the study. Metagenomic profiling of fecal microbiota and gut microbe-derived EVs in stool was performed using 16S ribosomal DNA sequencing. Relative abundance, evenness, and diversity in both the gut microbiota and gut microbe-derived EVs were analyzed. Additionally, microbial composition changes according to the stage and location of CRC were analyzed. Microbial composition was significantly changed in CRC subjects compared to control subjects, with evenness and diversity significantly lower in the fecal microbiota of CRC subjects. Gut microbe-derived EVs of stool demonstrated significant differences in the microbial composition, evenness, and diversity in CRC subjects compared to the control subjects. Additionally, microbial composition, evenness, and diversity significantly changed in late CRC subjects compared to early CRC subjects with both fecal microbiota and gut microbe-derived EVs. Alistipes-derived EVs could be novel biomarkers for diagnosing CRC and predicting CRC stages. Ruminococcus 2-derived EVs significantly decreased in distal CRC subjects than in proximal CRC subjects. Gut microbe-derived EVs in CRC had a distinct microbial composition compared to the controls. Profiling of microbe-derived EVs may offer a novel biomarker for detecting and predicting CRC prognosis.</p>
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页数：14
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