Expression and purification of exendin-4, a GLP-1 receptor agonist, in Escherichia coli

被引：20
作者：
Yin, XP [1 ]
Wei, DZ [1 ]
Yi, LN [1 ]
Tao, XY [1 ]
Ma, YS [1 ]
机构：
[1] E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2005年 / 41卷 / 02期
基金：
国家高技术研究发展计划(863计划);
关键词：
exendin-4; expression; glucose-lowering action;
D O I：
10.1016/j.pep.2004.10.014
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Exendin-4 is a 39 amino acid peptide isolated from salivary secretions of Gila monster (Heloderma suspectrum). It shows 53% sequence similarity to glucagon-like peptide-1 (GLP-1), which is evaluated for the regulation of plasma glucose in type 2 diabetes. Exendin-4 is a potent and long-acting agonist of GLP-1 receptor. In the present Study, the exendin-4 gene obtained by PCR with an enterokinase site at N-terminus and a termination codon at C-terminus was expressed in Escherichia coli strain BL21 (DE3) harboring pET32a(+). The fusion protein was purified by chromatography on Ni-NTA-agarose column. Recombinant exendin-4 was obtained by enterokinase cleavage of the fusion protein and subsequent purification. The yield of recombinant exendin-4 was 3.15 mg/10g bacteria. The obtained recombinant exendin-4 shows glucose-lowering action in vivo. (c) 2004 Elsevier Inc. All rights reserved.
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收藏
页码：259 / 265
页数：7
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