The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-β1/Smads signaling pathway

被引：22

作者：

Yang, Hong-Xia ^{[1
]}

Xu, Geng-Rui ^{[1
]}

Zhang, Chuang ^{[1
]}

Sun, Jia-Huan ^{[2
]}

Zhang, Yue ^{[1
,3
]}

Song, Jun-Na ^{[4
]}

Li, Yun-Feng ^{[1
,3
]}

Liu, Yu ^{[1
,3
]}

Li, Ai-Ying ^{[1
,3
]}

机构：

[1] Hebei Univ Chinese Med, Coll Basic Med, Dept Biochem & Mol Biol, 3 Xing Yuan Rd, Shijiazhuang 050200, Hebei, Peoples R China

[2] Hebei Univ Chinese Med, Coll Integrat Chinese & Western Med, Dept Med Lab Sci, Shijiazhuang 050200, Hebei, Peoples R China

[3] Hebei Key Lab Chinese Med Res Cardiocerebrovasc D, Shijiazhuang 050091, Hebei, Peoples R China

[4] Hebei Univ Chinese Med, Coll Pharm, Dept Med Plant, Shijiazhuang 050200, Hebei, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2020年 / 45卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Gentianella acuta; isoproterenol; myocardial fibrosis; transforming growth factor-beta 1; mothers against decapentaplegic homolog signaling pathway; TGF-BETA; CARDIAC FIBROSIS; XANTHONES;

D O I：

10.3892/ijmm.2019.4410

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Gentianella acuta (G. acuta) is one of the most commonly used herbs in Chinese Mongolian medicine for the treatment of heart disease. Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlying anti-fibrotic mechanism of G. acuta was further elucidated. Histopathological changes in the heart were observed by hematoxylineosin, Masson trichrome and wheat germ agglutinin staining. Relevant molecular events were investigated using immunohistochemistry and western blotting. The results revealed that G. acuta caused improvements in myocardial injury and fibrosis. G. acuta also inhibited collagens I and III and alpha -smooth muscle actin production in heart tissue. G. acuta downregulated the expression of transforming growth factor beta 1 (TGF-beta 1) and notably inhibited the levels of phosphorylation of TGF-beta receptors I and II. Furthermore, G. acuta caused downregulation of the intracellular mothers against decapentaplegic homolog (Smads)2 and 4 expression and inhibited Smads2 and 3 phosphorylation. The results further demonstrated that the mechanism underlying anti-myocardial fibrosis effects of G. acuta was based upon the suppression of the TGF-beta 1/Smads signaling pathway. Therefore, G. acuta may be a potential therapeutic agent for ameliorating myocardial fibrosis.

引用

页码：223 / 233

页数：11

共 43 条

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