Genome-wide investigation and comparative expression profiling reveal R2R3-MYB genes involved in Sclerotinia sclerotiorum defence in tomato

被引：8

作者：

Wong, Gwo Rong ^{[1
]}

Latif, Siti Nur Fatihah Binti Abd ^{[1
]}

Mazumdar, Purabi ^{[1
]}

机构：

[1] Univ Malaya, Ctr Res Biotechnol Agr, Kuala Lumpur 50603, Malaysia

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2022年 / 121卷

关键词：

MYB; Sclerotinia sclerotiorum; Biotic stress; Defence; Tomato; MYB TRANSCRIPTION FACTOR; CUTICULAR WAX BIOSYNTHESIS; ABIOTIC STRESS RESPONSES; DISEASE RESISTANCE; METHYL JASMONATE; FUNGAL-INFECTION; POWDERY MILDEW; GRAPE BERRIES; CHERRY TOMATO; CELL-DEATH;

D O I：

10.1016/j.pmpp.2022.101873

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

R2R3-MYB genes are involved in several plant regulatory processes, including defence responses. However, little is known about the role of R2R3-MYB genes involved in Sclerotia sclerotiorum defence response in tomato plants. In this study, 26 tomato R2R3-MYB genes (SlMYB) with putative defence functions were identified. The promoter analysis showed other than MYB binding domain (MBS/MYB recognition, MYB-binding site/MYB-like sequence), 65.38% of selected SlMYB contains CGTCA-motif, 34.62% contains TC-rich and TCA-elements, 30.77% contains W-box, 30.77% contains WUN motif. Analysis of susceptibility among tomato cultivars showed "Red Rock' is highly susceptible (88.66%), followed by "Tropical' (61.49%) and "Super Star' (22.88%). Analysis of SlMYBs showed differential expression among the cultivars at 7 and 21 days post-inoculation. Relative expression of the selected SlMYBs among the cultivars showed strong expression of SlMYBs (24 out of 26 MYBs) in Super Star followed by tropical and Red Rock. Defence enzymes; chitinase, beta-1-3-glucanases, phenylalanine ammonialyase and polyphenol oxidases were also found to show a similar pattern, suggesting an association of SlMYBs expression with the activity of defence enzymes. Five SlMYBs (9, 13, 14, 15 and 112) showed strong upregulation in Super Star inoculated S. sclerotiorum. This study provides fundamental insights into the involvement of SlMYBs in regulating the response of tomato against S. sclerotiorum.

引用

页数：13

共 109 条

[1] Crosstalk between biotic and abiotic stress responses in tomato is mediated by the AIM1 transcription factor [J].

AbuQamar, Synan ;

Luo, Hongli ;

Laluk, Kristin ;

Mickelbart, Michael V. ;

Mengiste, Tesfaye .

PLANT JOURNAL, 2009, 58 (02) :347-360

[2] Endogenous siRNA and miRNA targets identified by sequencing of the Arabidopsis degradome [J].

Addo-Quaye, Charles ;

Eshoo, Tifani W. ;

Bartel, David P. ;

Axtell, Michael J. .

CURRENT BIOLOGY, 2008, 18 (10) :758-762

[3] Comparison of Accumulation of Stilbene Compounds and Stilbene Related Gene Expression in Two Grape Berries Irradiated with Different Light Sources [J].

Ahn, Soon Young ;

Kim, Seon Ae ;

Choi, Seong-Jin ;

Yun, Hae Keun .

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, 2015, 56 (01) :36-43

[4] A novel TaMYB4 transcription factor involved in the defence response against Puccinia striiformis f. sp tritici and abiotic stresses [J].

Al-Attala, M. Nashaat ;

Wang, Xiaojie ;

Abou-Attia, M. A. ;

Duan, Xiaoyuan ;

Kang, Zhensheng .

PLANT MOLECULAR BIOLOGY, 2014, 84 (4-5) :589-603

[5] Overexpression of a rice heme activator protein gene (OsHAP2E) confers resistance to pathogens, salinity and drought, and increases photosynthesis and tiller number [J].

Alam, Md Mahfuz ;

Tanaka, Toru ;

Nakamura, Hidemitsu ;

Ichikawa, Hiroaki ;

Kobayashi, Kappei ;

Yaeno, Takashi ;

Yamaoka, Naoto ;

Shimomoto, Kota ;

Takayama, Kotaro ;

Nishina, Hiroshige ;

Nishiguchi, Masamichi .

PLANT BIOTECHNOLOGY JOURNAL, 2015, 13 (01) :85-96

[6] Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens [J].

Ali, Niaz ;

Chen, Hua ;

Zhang, Chong ;

Khan, Shahid Ali ;

Gandeka, Mamadou ;

Xie, Dongyang ;

Zhuang, Weijian .

GENES, 2020, 11 (03)

[7] Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance [J].

Ali, Sajad ;

Ganai, Bashir Ahmad ;

Kamili, Azra N. ;

Bhat, Ajaz Ali ;

Mir, Zahoor Ahmad ;

Bhat, Javaid Akhter ;

Tyagi, Anshika ;

Islam, Sheikh Tajamul ;

Mushtaq, Muntazir ;

Yadav, Prashant ;

Rawat, Sandhya ;

Grover, Anita .

MICROBIOLOGICAL RESEARCH, 2018, 212 :29-37

[8] Genetic analysis reveals functional redundancy and the major target genes of the Arabidopsis miR159 family [J].

Allen, Robert S. ;

Li, Junyan ;

Stahle, Melissa I. ;

Dubroue, Aurelie ;

Gubler, Frank ;

Millar, Anthony A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (41) :16371-16376

[9] MYB transcription factor genes as regulators for plant responses: an overview [J].

Ambawat, Supriya ;

Sharma, Poonam ;

Yadav, Neelam R. ;

Yadav, Ram C. .

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2013, 19 (03) :307-321

[10] Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea [J].

Amselem, Joelle ;

Cuomo, Christina A. ;

van Kan, Jan A. L. ;

Viaud, Muriel ;

Benito, Ernesto P. ;

Couloux, Arnaud ;

Coutinho, Pedro M. ;

de Vries, Ronald P. ;

Dyer, Paul S. ;

Fillinger, Sabine ;

Fournier, Elisabeth ;

Gout, Lilian ;

Hahn, Matthias ;

Kohn, Linda M. ;

Lapalu, Nicolas ;

Plummer, Kim M. ;

Pradier, Jean-Marc ;

Quevillon, Emmanuel ;

Sharon, Amir ;

Simon, Adeline ;

ten Have, Arjen ;

Tudzynski, Bettina ;

Tudzynski, Paul ;

Wincker, Patrick ;

Andrew, Marion ;

Anthouard, Veronique ;

Beever, Ross E. ;

Beffa, Rolland ;

Benoit, Isabelle ;

Bouzid, Ourdia ;

Brault, Baptiste ;

Chen, Zehua ;

Choquer, Mathias ;

Collemare, Jerome ;

Cotton, Pascale ;

Danchin, Etienne G. ;

Da Silva, Corinne ;

Gautier, Angelique ;

Giraud, Corinne ;

Giraud, Tatiana ;

Gonzalez, Celedonio ;

Grossetete, Sandrine ;

Gueldener, Ulrich ;

Henrissat, Bernard ;

Howlett, Barbara J. ;

Kodira, Chinnappa ;

Kretschmer, Matthias ;

Lappartient, Anne ;

Leroch, Michaela ;

Levis, Caroline .

PLOS GENETICS, 2011, 7 (08)

← 1 2 3 4 5 6 7 8 9 10 →