Artificial testing targets with controllable blur for adaptive optics microscopes

被引：4

作者：

Hattori, Masayuki ^{[1
]}

Tamada, Yosuke ^{[2
,3
]}

Murata, Takashi ^{[2
,3
]}

Oya, Shin ^{[4
]}

Hasebe, Mitsuyasu ^{[2
,3
]}

Hayano, Yutaka ^{[5
,6
]}

Kamei, Yasuhiro ^{[1
,3
]}

机构：

[1] Natl Inst Basic Biol, Spect & Bioimaging Facil, Okazaki, Aichi, Japan

[2] Natl Inst Basic Biol, Div Evolutionary Biol, Okazaki, Aichi, Japan

[3] Grad Univ Adv Studies, Sch Life Sci, Okazaki, Aichi, Japan

[4] Natl Astron Observ Japan, TMT J Project Off, Mitaka, Tokyo, Japan

[5] Natl Astron Observ Japan, Adv Technol Ctr, Mitaka, Tokyo, Japan

[6] Grad Univ Adv Studies, Sch Phys Sci, Mitaka, Tokyo, Japan

来源：

OPTICAL ENGINEERING | 2017年 / 56卷 / 08期

关键词：

adaptive optics; microscopy; imaging through turbulent media; ABERRATION CORRECTION; PUPIL-SEGMENTATION; ILLUMINATION;

D O I：

10.1117/1.OE.56.8.080502

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This letter proposes a method of configuring a testing target to evaluate the performance of adaptive optics microscopes. In this method, a testing slide with fluorescent beads is used to simultaneously determine the point spread function and the field of view. The point spread function is reproduced to simulate actual biological samples by etching a microstructure on the cover glass. The fabrication process is simplified to facilitate an onsite preparation. The artificial tissue consists of solid materials and silicone oil and is stable for use in repetitive experiments. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：4

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