Model-based strategy for cell culture seed train layout verified at lab scale

被引：15

作者：

Kern, Simon ^{[1
]}

Platas-Barradas, Oscar ^{[2
]}

Poertner, Ralf ^{[2
]}

Frahm, Bjoern ^{[1
]}

机构：

[1] Ostwestfalen Lippe Univ Appl Sci, Fac Life Sci Technol, Biotechnol & Bioproc Engn, Liebigstr 87, D-32657 Lemgo, Germany

[2] Hamburg Univ Technol, Inst Bioproc & Biosyst Engn, Denickestr 15, D-21073 Hamburg, Germany

来源：

CYTOTECHNOLOGY | 2016年 / 68卷 / 04期

关键词：

Seed train; Design; Layout; Optimization; Modeling; Suspension cell cultures; Space-Time-Yield; FED-BATCH CULTIVATION; HYBRIDOMA CELLS; METABOLISM; GROWTH;

D O I：

10.1007/s10616-015-9858-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN (AAT) and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN (AAT) cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.

引用

页码：1019 / 1032

页数：14

共 21 条

[1] Alahari A., 2009, BioProcess Int, V7, P48
[2] Evaluation of criteria for bioreactor comparison and operation standardization for mammalian cell culture
Barradas, Oscar Platas
Jandt, Uwe
Linh Da Minh Phan
Villanueva, Mario E.
Schaletzky, Martin
Rath, Alexander
Freund, Susann
Reichl, Udo
Skerhutt, Eva
Scholz, Sebastian
Noll, Thomas
Sandig, Volker
Poertner, Ralf
Zeng, An-Ping
[J]. ENGINEERING IN LIFE SCIENCES, 2012, 12 (05): : 518 - 528
[3] Industrial choices for protein production by large-scale cell culture
Chu, L
Robinson, DK
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (02) : 180 - 187
[4] Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis
Frahm, B
Lane, P
Märkl, H
Pörtner, R
[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2003, 26 (01) : 1 - 10
[5] Adaptive, model-based control by the Open-Loop-Feedback-Optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells
Frahm, B
Lane, P
Atzert, H
Munack, A
Hoffmann, M
Hass, VC
Pörtner, R
[J]. BIOTECHNOLOGY PROGRESS, 2002, 18 (05) : 1095 - 1103
[6] Frahm B, 2014, ANIMAL CELL BIOTECHN
[7] A new seed-train expansion method for recombinant mammalian cell lines
Heidemann, R
Mered, M
Wang, DQ
Gardner, B
Zhang, C
Michaels, J
Henzler, HJ
Abbas, N
Konstantinov, K
[J]. CYTOTECHNOLOGY, 2002, 38 (1-2) : 99 - 108
[8] Heidemann R, 2003, US Patent, Patent No. [2003/0113915 A1, 20030113915]
[9] Jang DJ, 2000, BIOCHEM ENG J, V4, P153
[10] LI F, 2006, BIOPROCESS J, V5, P16

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