Comparative transcriptome analysis reveals that deletion of CheY influences gene expressions of ABC transports and metabolism in Haemophilus parasuis

被引:4
作者
He, Lvqin [1 ]
Yan, Xuefeng [2 ]
Dai, Ke [3 ]
Wen, Xintian [3 ]
Cao, Sanjie [3 ]
Huang, Xiaobo [3 ]
Wu, Rui [3 ]
Zhao, Qin [3 ]
Huang, Yong [3 ]
Yan, Qigui [3 ]
Ma, Xiaoping [3 ]
Han, Xinfeng [3 ]
Wen, Yiping [3 ]
机构
[1] Southwest Med Univ, Technol Dept, Expt Anim Ctr, Luzhou, Peoples R China
[2] Southwest Med Univ, Technol Dept, Luzhou, Peoples R China
[3] Sichuan Agr Univ, Coll Vet Med, Chengdu, Sichuan, Peoples R China
关键词
Haemophilus parasuis; CheY; RNA sequencing; Regulatory network; BIOFILM FORMATION; RESISTANCE; VIRULENCE; STRESS; COLONIZATION; REGULATOR; BINDING; QSEBC; MSBA;
D O I
10.1007/s10142-021-00800-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Haemophilus (Glaesserella) parasuis is a commensal bacterium that causes Glasser's disease (GD) in swine. As a global transcriptional factor, CheY regulates the expression of hundreds of genes in H. parasuis. In this study, we measured changes in gene expression at the whole transcriptome level using RNAseq. We identified 2058 co-expressed genes, and found 624 differentially expressed genes (q < 0.05) in Delta cheY and SC1401. Several important GO annotations and signaling pathways were identified. RNA-seq results were assembled according to the reference genome, compared with the annotated gene model, and 12 new transcriptional regions were found. Finally, q-PCR results validated the RNA-seq results with 8 randomly selected genes. The present study indicated that CheY is mainly involved in the regulation of ABC transport, oxidative phosphorylation, and beta-Lactam resistance. We draw the regulatory network of CheY, which offers greater insight into the regulatory mechanism of CheY in H.parasuis.
引用
收藏
页码:695 / 707
页数:13
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