Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response

被引:22
作者
Liu, Ming-Kun [1 ]
Lin, Jhe-Jhih [1 ]
Chen, Chung-Yung [2 ]
Kuo, Szu-Cheng [3 ]
Wang, Yu-Ming [3 ]
Chan, Hong-Lin [1 ]
Wu, Tzong Yuan [2 ,4 ]
机构
[1] Natl Tsing Hua Univ, Inst Bioinformat & Struct Biol, Hsinchu 300, Taiwan
[2] Chung Yuan Christian Univ, Dept Biosci Technol, Chungli 320, Taiwan
[3] Inst Prevent Med, Natl Def Med Ctr, Taipei 237, Taiwan
[4] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung 404, Taiwan
关键词
baculovirus; BacMam; DNA damage response; topoisomerase; p53; PROTEIN EXPRESSION; HEPATOCELLULAR-CARCINOMA; INSECT; DELIVERY; VECTORS; ENTRY; HEPATOCYTES; SEQUENCE; BREAKS;
D O I
10.3390/ijms17060931
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
BacMam is an insect-derived recombinant baculovirus that can deliver genes into mammalian cells. BacMam vectors carrying target genes are able to enter a variety of cell lines by endocytosis, but the level of expression of the transgene depends on the cell line and the state of the transduced cells. In this study, we demonstrated that the DNA damage response (DDR) could act as an alternative pathway to boost the transgene(s) expression by BacMam and be comparable to the inhibitors of histone deacetylase. Topoisomerase II (Top II) inhibitor-induced DDR can enhance the CMV-IE/enhancer mediated gene expression up to 12-fold in BacMam-transduced U-2OS cells. The combination of a Top II inhibitor, VM-26, can also augment the killing efficiency of a p53-expressing BacMam vector in U-2OS osteosarcoma cells. These results open a new avenue to facilitate the application of BacMam for gene delivery and therapy.
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页数:15
相关论文
共 47 条
[1]   Baculovirus: an Insect-derived Vector for Diverse Gene Transfer Applications [J].
Airenne, Kari J. ;
Hu, Yu-Chen ;
Kost, Thomas A. ;
Smith, Richard H. ;
Kotin, Robert M. ;
Ono, Chikako ;
Matsuura, Yoshiharu ;
Wang, Shu ;
Yla-Herttuala, Seppo .
MOLECULAR THERAPY, 2013, 21 (04) :739-749
[2]   BacMam technology and its application to drug discovery [J].
Ames, Robert S. ;
Kost, Thomas A. ;
Condreay, J. Patrick .
EXPERT OPINION ON DRUG DISCOVERY, 2007, 2 (12) :1669-1681
[3]   BacMam recombinant Baculoviruses in G protein-coupled receptor drug discovery [J].
Ames, RS ;
Fornwald, JA ;
Nuthulaganti, P ;
Trill, JJ ;
Foley, JJ ;
Buckley, PT ;
Kost, TA ;
Wu, ZN ;
Romanos, MA .
RECEPTORS & CHANNELS, 2004, 10 (3-4) :99-107
[4]   THE COMPLETE DNA-SEQUENCE OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].
AYRES, MD ;
HOWARD, SC ;
KUZIO, J ;
LOPEZFERBER, M ;
POSSEE, RD .
VIROLOGY, 1994, 202 (02) :586-605
[5]   Baculovirus-mediated gene transfer into mammalian cells [J].
Boyce, FM ;
Bucher, NLR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (06) :2348-2352
[6]   Suppression of hepatocellular carcinoma by baculovirus-mediated expression of long non-coding RNA PTENP1 and MicroRNA regulation [J].
Chen, Chiu-Ling ;
Tseng, Yen-Wen ;
Wu, Jaw-Ching ;
Chen, Guan-Yu ;
Lin, Kuan-Chen ;
Hwang, Shiaw-Min ;
Hu, Yu-Chen .
BIOMATERIALS, 2015, 44 :71-81
[7]   Baculovirus-Mediated miRNA Regulation to Suppress Hepatocellular Carcinoma Tumorigenicity and Metastasis [J].
Chen, Chiu-Ling ;
Wu, Jaw-Ching ;
Chen, Guan-Yu ;
Yuan, Pei-Hsiang ;
Tseng, Yen-Wen ;
Li, Kuei-Chang ;
Hwang, Shiaw-Min ;
Hu, Yu-Chen .
MOLECULAR THERAPY, 2015, 23 (01) :79-88
[8]   Development of Hybrid Baculovirus Vectors for Artificial MicroRNA Delivery and Prolonged Gene Suppression [J].
Chen, Chiu-Ling ;
Luo, Wen-Yi ;
Lo, Wen-Hsin ;
Lin, Kun-Ju ;
Sung, Li-Yu ;
Shih, Yung-Shen ;
Chang, Yu-Han ;
Hu, Yu-Chen .
BIOTECHNOLOGY AND BIOENGINEERING, 2011, 108 (12) :2958-2967
[9]   Membrane penetrating peptides greatly enhance baculovirus transduction efficiency into mammalian cells [J].
Chen, Hong-Zhang ;
Wu, Carol P. ;
Chao, Yu-Chan ;
Liu, Catherine Yen-Yen .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 405 (02) :297-302
[10]   Development of a bi-cistronic baculovirus expression vector by the Rhopalosiphum padi virus 5′ internal ribosome entry site [J].
Chen, YJ ;
Chen, WS ;
Wu, TY .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 335 (02) :616-623